Compartmentalized microchannel array for high-throughput analysis of single cell polarized growth and dynamics
| Autor: | Scott E. Baker, Craig Szymanski, Erin L. Bredeweg, Bingwen Liu, James E. Evans, Tao Geng, Galya Orr, Ryan T. Kelly |
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| Jazyk: | angličtina |
| Rok vydání: | 2015 |
| Předmět: |
Microfluidics
Cell Population Hyphae Article Neurospora crassa law.invention law Gene Expression Regulation Fungal medicine Morphogenesis Compartment (development) education education.field_of_study Multidisciplinary Microchannel Microscopy Video biology Ecology Dynamics (mechanics) Lab-on-a-chip Microfluidic Analytical Techniques biology.organism_classification Microarray Analysis Carbon medicine.anatomical_structure Microscopy Fluorescence Biophysics |
| Zdroj: | Scientific Reports |
| ISSN: | 2045-2322 |
| DOI: | 10.1038/srep16111 |
| Popis: | Interrogating polarized growth is technologically challenging due to extensive cellular branching and uncontrollable environmental conditions in conventional assays. Here we present a robust and high-performance microfluidic system that enables observations of polarized growth with enhanced temporal and spatial control over prolonged periods. The system has built-in tunability and versatility to accommodate a variety of scientific applications requiring precisely controlled environments. Using the model filamentous fungus, Neurospora crassa, our microfluidic system enabled direct visualization and analysis of cellular heterogeneity in a clonal fungal cell population, nuclear distribution and dynamics at the subhyphal level and quantitative dynamics of gene expression with single hyphal compartment resolution in response to carbon source starvation and exchange. Although the microfluidic device is demonstrated on filamentous fungi, the technology is immediately extensible to a wide array of other biosystems that exhibit similar polarized cell growth, with applications ranging from bioenergy production to human health. |
| Databáze: | OpenAIRE |
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