Co-immobilization multienzyme nanoreactor with co-factor regeneration for conversion of CO2
| Autor: | Sizhu Ren, Yuxiao Feng, Muhammad Bilal, Jiandong Cui, Shiru Jia, Yunhong Jiang, Ziyuan Wang |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
0303 health sciences
Ion exchange Bioconversion 02 engineering and technology General Medicine Nanoreactor C700 021001 nanoscience & nanotechnology Formate dehydrogenase Biochemistry Polyelectrolyte 03 medical and health sciences chemistry.chemical_compound Chemical engineering chemistry Structural Biology Yield (chemistry) Formate 0210 nano-technology Molecular Biology Dissolution 030304 developmental biology |
| Zdroj: | International Journal of Biological Macromolecules. 155:110-118 |
| ISSN: | 0141-8130 |
| Popis: | Multienzymatic conversion of carbon dioxide (CO2) into chemicals has been extensively studied. However, regeneration and reuse of co-factor are still the main problems for the efficient conversion of CO2. In this study, a nanoscale multienzyme reactor was constructed by encapsulating simultaneously carbonic anhydrase (CA), formate dehydrogenase (FateDH), co-factor (NADH), and glutamate dehydrogenases (GDH) into ZIF-8. In the multienzyme reactors, cationic polyelectrolyte (polyethyleneimine, PEI) was doped in the ZIF-8 by dissolving it in the precursors of ZIF-8. Co-factor (NADH) was anchored in ZIF-8 by ion exchange between PEI (positive charge) and co-factor (negative charge), and regenerated through GDH embedded in the ZIF-8, thus keeping high activity of FateDH. Activity recovery of FateDH in the multienzyme reactors reached 50%. Furthermore, the dissolution of CO2 in the reaction solution was increased significantly by the combination of CA and ZIF-8. As a result, the nanoscale multienzyme reactor exhibited superior capacity for conversion of CO2 to formate. Compared with free multienzyme system, formate yield was increased 4.6-fold by using the nanoscale multienzyme reactor. Furthermore, the nanoscale multienzyme reactor still retained 50% of its original productivity after 8 cycles, indicating excellent reusability. |
| Databáze: | OpenAIRE |
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