Assessing the Interactions of Auristatin Derivatives with Mixed Phospholipid–Sodium Dodecyl Sulfate Aggregate Dispersions
| Autor: | Filip S. Ekholm, Susanne K. Wiedmer, Suvi-Katriina Ruokonen |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
Liposome
Chromatography Vesicle Phospholipid 02 engineering and technology Surfaces and Interfaces 010402 general chemistry 021001 nanoscience & nanotechnology Condensed Matter Physics 01 natural sciences Micelle Article 0104 chemical sciences chemistry.chemical_compound Pulmonary surfactant Monomethyl auristatin E chemistry Dynamic light scattering Electrochemistry lipids (amino acids peptides and proteins) General Materials Science Sodium dodecyl sulfate 0210 nano-technology Spectroscopy |
| Zdroj: | Langmuir |
| ISSN: | 1520-5827 0743-7463 |
| Popis: | The aim of this study was to assess what properties of the pseudostationary phases in electrokinetic capillary chromatography affect the interactions between monomethyl auristatin E (MMAE) and hydrophilically modified structural analogues thereof with various lipophilic phases. MMAE is a widely used cytotoxic agent in antibody–drug conjugates (ADC), which are used as selective biopharmaceutical drugs in the treatment of cancers. MMAE and its derivatives are highly lipophilic, yet they fail to interact with biomimicking phosphatidylcholine–phosphatidylserine liposomes. To reveal what properties affect the interaction of the auristatin derivatives with cell plasma membrane-mimicking vesicles, capillary electrokinetic chromatography was used with four different types of micellar and vesicular pseudostationary phases: pure vesicles, mixed vesicles, mixed micelles, and pure micelles. Vesicular phases were composed of pure phospholipids [dimyristoylphosphatidylcholine (DMPC) and dilauroylphosphatidylcholine (DLPC)] and phospholipid–surfactant mixtures [sodium dodecyl sulfate, (SDS) with DMPC and DLPC] while the micellar phases comprised pure surfactant (SDS) and surfactant–phospholipid mixtures (SDS–DMPC and SDS–DLPC). In addition, differential scanning calorimetry and dynamic light scattering were used to monitor the aggregate composition. Our data shows that the interaction between hydrophobic auristatin derivatives and hydrophobic pseudostationary phases critically depends on the type, size, and hydrogen bonding capability of the pseudostationary phases. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|