Detection and Identification of Gastrointestinal Lactobacillus Species by Using Denaturing Gradient Gel Electrophoresis and Species-Specific PCR Primers
| Autor: | Diane M. Loach, Anu Tilsala-Timisjärvi, Gerald W. Tannock, Sureelak Rodtong, Karen Munro, Jens Walter, Tapani Alatossava |
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| Rok vydání: | 2000 |
| Předmět: |
DNA
Bacterial Ribosomal Intergenic Spacer analysis Biology DNA Ribosomal Polymerase Chain Reaction Applied Microbiology and Biotechnology Microbiology law.invention Mice Species Specificity law RNA Ribosomal 16S Lactobacillus Animals Humans Polymerase chain reaction DNA Primers Ecology Stomach Genes rRNA Spacer DNA Ribosomal RNA biology.organism_classification 16S ribosomal RNA Molecular biology RNA Ribosomal 23S GenBank Food Microbiology Electrophoresis Polyacrylamide Gel Temperature gradient gel electrophoresis Food Science Biotechnology |
| Zdroj: | Applied and Environmental Microbiology. 66:297-303 |
| ISSN: | 1098-5336 0099-2240 |
| DOI: | 10.1128/aem.66.1.297-303.2000 |
| Popis: | Denaturing gradient gel electrophoresis (DGGE) of DNA fragments obtained by PCR amplification of the V2-V3 region of the 16S rRNA gene was used to detect the presence of Lactobacillus species in the stomach contents of mice. Lactobacillus isolates cultured from human and porcine gastrointestinal samples were identified to the species level by using a combination of DGGE and species-specific PCR primers that targeted 16S-23S rRNA intergenic spacer region or 16S rRNA gene sequences. The identifications obtained by this approach were confirmed by sequencing the V2-V3 region of the 16S rRNA gene and by a BLAST search of the GenBank database. |
| Databáze: | OpenAIRE |
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