Antibacterial activity of soil bacteria isolated from Kochi, India and their molecular identification
| Autor: | Dorairaj Sudarsanam, Davis Gislin, Gnanaprakasam Antony Raj, Kathirvelu Baskar |
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| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
lcsh:QH426-470 Bacillus amyloliquefaciens lcsh:Biotechnology 030106 microbiology medicine.disease_cause Isolates Microbiology 03 medical and health sciences lcsh:TP248.13-248.65 medicine General Materials Science Agar diffusion test 16S rRNA biology Microbial/industrial Biotechnology Pseudomonas aeruginosa Soil bacteria Antibiosis biology.organism_classification 16S ribosomal RNA lcsh:Genetics PCR Enterococcus GenBank Bacteria |
| Zdroj: | Journal of Genetic Engineering & Biotechnology Journal of Genetic Engineering and Biotechnology, Vol 16, Iss 2, Pp 287-294 (2018) |
| ISSN: | 2090-5920 |
| Popis: | The present study, deal about the antibiosis activity of soil bacteria, isolated from 10 different locations of rhizosphere and diverse cultivation at Kochi, Kerala, India. The bacteria were isolated by standard serial dilution plate techniques. Morphological characterization of the isolate was done by Gram’s staining and found that all of them gram positive. Isolated bacteria were tested against 6 human pathogens viz., Escherichia coli, Enterococcus sp., Pseudomonas aeruginosa, Klebsiella pneumoniae, Staphylococcus aureus and Acinetobacter sp. Primary screening was carried out by perpendicular streaking and seed overlay method. Based on the result of primary screening most potential isolates of S1A1 and S7A3 were selected for secondary screening. Both the isolates showed positive results against Enterococcus sp. and S.aureus. The maximum antagonistic activity of 20.98 and 27.08 mm zone of inhibition was recorded at S1A1 against Enterococcus sp. and S. aureus respectively, at 180 µl concentration. Molecular identification was carried out by 16S rRNA sequence. The 16S rRNA was amplified from the DNA samples by using PCR. The amplified 16S rRNA PCR products were purified and sequenced. The sequences were subjected to NCBI BLAST. The isolates S1A1 and S7A3 BLAST results showed 99% and 95% respectively, similarity with the available database sequence of Bacillus amyloliquefaciens. The sequences were deposited in GenBank and the accession numbers {"type":"entrez-nucleotide","attrs":{"text":"KY864390","term_id":"1172930734","term_text":"KY864390"}}KY864390 (S1A1) and {"type":"entrez-nucleotide","attrs":{"text":"KY880975","term_id":"1173392218","term_text":"KY880975"}}KY880975 (S7A3) were obtained. |
| Databáze: | OpenAIRE |
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