Determination of Ethanol Content in Kombucha Using Headspace Gas Chromatography with Mass Spectrometry Detection: Single-Laboratory Validation
| Autor: | Jamie Finley, Paula N. Brown, Jake Robertson, Michael Chan, Hong Sy |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
AcademicSubjects/SCI01140
Canada Kombucha Accuracy and precision Food Chemical Contaminants AcademicSubjects/SCI01060 AcademicSubjects/SCI00030 Alcohol AcademicSubjects/SCI01180 Mass spectrometry Gas Chromatography-Mass Spectrometry Mass Spectrometry Analytical Chemistry chemistry.chemical_compound Humans Environmental Chemistry Child Mathematics Pharmacology Detection limit Chromatography Ethanol chemistry Ethanol content Fermentation AcademicSubjects/SCI00980 Gas chromatography Laboratories Agronomy and Crop Science Food Science |
| Zdroj: | Journal of AOAC International |
| ISSN: | 1944-7922 1060-3271 |
| DOI: | 10.1093/jaoacint/qsaa094 |
| Popis: | Background Kombucha is a fermented beverage made with tea, sugar, and a symbiotic colony of bacteria and yeast that is usually marketed as a non-alcoholic beverage. Products must contain Objective Given the public safety concerns and legal requirements associated with the level of alcohol within Kombucha beverages, there is a need for accurate and reliable methods. Herein we describe the validation of a sensitive, rapid, and simple Headspace Gas Chromatographic method with mass spectrometric detection for determining ethanol in Kombucha. Methods Method performance characteristics measured included linearity, accuracy, precision, limit of detection (LOD), and limit of quantification (LOQ) as per AOAC International guideline Appendix K Part 1. Performance was evaluated against the AOAC Standard Method Performance Requirements 2016.001 for determination of ethanol in Kombucha. Results The linear dynamic range for this method was confirmed over the range of 0.025 to 2.47% ABV. The LOD and LOQ were determined to be 0.0002% and 0.002% ABV, respectively. With a spike recovery of 102% for accuracy and precision of RSDr ≤ 4% the method met the SMPR requirements within the analytical range. Conclusions The results of this validation study demonstrated the method is fit for the purpose of quantifying ethanol in Kombucha and is suitable for rapid and easy integration by laboratories to ensure that regulatory requirements are met. |
| Databáze: | OpenAIRE |
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