Evolutionarily conserved sequences on human chromosome 21
| Autor: | John B. Sheehan, Jan Fang Cheng, Xiyin Chen, Nila Patil, Roya Hosseini, Renee Stokowski, David Cox, Kelly A. Frazer, Stephen P. A. Fodor |
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| Rok vydání: | 2001 |
| Předmět: |
Genetics
Chromosomes Artificial Bacterial Chromosomes Human Pair 21 Genome project DNA Biology ENCODE Trans-regulatory element Sensitivity and Specificity Synteny DNA sequencing Conserved non-coding sequence Conserved sequence Evolution Molecular Mice Dogs Genes Overlapping Animals Humans Human genome Chromosome 21 Genetics (clinical) Conserved Sequence Oligonucleotide Array Sequence Analysis |
| Zdroj: | Frazer, Kelly A.; Sheehan, John B.; Stokowski, Renee P.; Chen, Xiyin; Hosseini, Roya; Cheng, Jan-Fang; et al.(2001). Evolutionarily conserved sequences on human chromosome 21. Genome Research, 11(10). Lawrence Berkeley National Laboratory: Lawrence Berkeley National Laboratory. Retrieved from: http://www.escholarship.org/uc/item/81m073qw |
| ISSN: | 1088-9051 |
| Popis: | Comparison of human sequences with the DNA of other mammals is an excellent means of identifying functional elements in the human genome. Here we describe the utility of high-density oligonucleotide arrays as a rapid approach for comparing human sequences with the DNA of multiple species whose sequences are not presently available. High-density arrays representing ∼22.5 Mb of nonrepetitive human chromosome 21 sequence were synthesized and then hybridized with mouse and dog DNA to identify sequences conserved between humans and mice (human–mouse elements) and between humans and dogs (human–dog elements). Our data show that sequence comparison of multiple species provides a powerful empiric method for identifying actively conserved elements in the human genome. A large fraction of these evolutionarily conserved elements are present in regions on chromosome 21 that do not encode known genes. |
| Databáze: | OpenAIRE |
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