Expression ofObReceptor Splice Variants During Prenatal Development of the Mouse
| Autor: | Justine J. Cunningham, Shu-Cheng Chen, Richard J. Smeyne |
|---|---|
| Rok vydání: | 2000 |
| Předmět: |
RNA Splicing
Mesenchyme Receptors Cell Surface In situ hybridization Biology Biochemistry Mice Fetus Pregnancy Notochord medicine Animals splice RNA Messenger Receptor Molecular Biology In Situ Hybridization Genetics Messenger RNA Leptin receptor Embryogenesis Cell Biology Cell biology Mice Inbred C57BL medicine.anatomical_structure Receptors Leptin Female Carrier Proteins |
| Zdroj: | Journal of Receptors and Signal Transduction. 20:87-103 |
| ISSN: | 1532-4281 1079-9893 |
| DOI: | 10.3109/10799890009150039 |
| Popis: | In adult animals, signaling through the leptin receptor (OB-R) has been shown to play a critical role in fat metabolism. However, it is not known when these receptors are first expressed and what their role may be during embryonic development. To date, at least 6 splice variants of the OB-R have been identified. Although the function of each of these individual splice variants are unknown, only one of them, ob-rL ,encodes a receptor with a long intracellular domain that is implicated in OB-R signaling. In this study we have used in situ hybridization to examine the localization of OB-R splice variants during embryonic development of C57B1/6J mice. Using a probe, ob-r, that recognizes all of the splice variants, ob-r mRNA was found to be distributed in developing bone, mesenchyme, notochord and liver. In addition, epithelial structures including leptomeninges, choroid plexi and hair follicles also expressed ob-r. No ob-r mRNA was detected in the CNS. ob-rL, expression was only detected in notochord, bone and mesenchyme. The differential expression of these two mRNA isoforms suggests that the extracellular and intracellular domains of the OB receptor perform different biological functions. |
| Databáze: | OpenAIRE |
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