Evaluation of bone matrix gelatin/fibrin glue and chitosan/gelatin composite scaffolds for cartilage tissue engineering
| Autor: | X Y Zhao, Demin Kong, Jianning Zhang, Yanxia Shi, Xingwen Li, Qiaogen Zhang, Yong-Jing Gao, Zhenghui Wang, Jinquan Zhao, Jianqun Yan, Yintong Yang |
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| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
food.ingredient Bone Matrix macromolecular substances Matrix (biology) Gelatin Fibrin Chondrocyte Rats Sprague-Dawley Chitosan 03 medical and health sciences chemistry.chemical_compound Chondrocytes food Tissue engineering Adhesives Cell Adhesion Genetics medicine Animals Aggrecans Fibrin glue Collagen Type II Molecular Biology Cells Cultured Aggrecan Tissue Engineering Tissue Scaffolds biology technology industry and agriculture General Medicine Rats 030104 developmental biology medicine.anatomical_structure chemistry biology.protein Chondrogenesis Biomedical engineering |
| Zdroj: | Genetics and Molecular Research. 15 |
| ISSN: | 1676-5680 |
| DOI: | 10.4238/gmr.15038431 |
| Popis: | This study was designed to evaluate bone matrix gelatin (BMG)/fibrin glue and chitosan/gelatin composite scaffolds for cartilage tissue engineering. Chondrocytes were isolated from costal cartilage of Sprague-Dawley rats and seeded on BMG/fibrin glue or chitosan/gelatin composite scaffolds. After different in vitro culture durations, the scaffolds were subjected to hematoxylin and eosin, Masson's trichrome, and toluidine blue staining, anti-collagen II and anti-aggrecan immunohistochemistry, and scanning electronic microscopy (SEM) analysis. After 2 weeks of culture, chondrocytes were distributed evenly on the surfaces of both scaffolds. Cell numbers and the presence of extracellular matrix components were markedly increased after 8 weeks of culture, and to a greater extent on the chitosan/gelatin scaffold. The BMG/fibrin glue scaffold showed signs of degradation after 8 weeks. Immunofluorescence analysis confirmed higher levels of collagen II and aggrecan using the chitosan/gelatin scaffold. SEM revealed that the majority of cells on the surface of the BMG/fibrin glue scaffold demonstrated a round morphology, while those in the chitosan/gelatin group had a spindle-like shape, with pseudopodia. Chitosan/gelatin scaffolds appear to be superior to BMG/ fibrin glue constructs in supporting chondrocyte attachment, proliferation, and biosynthesis of cartilaginous matrix components. |
| Databáze: | OpenAIRE |
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