Phytochemical profile and α-glucosidase inhibitory activity of Sardinian Hypericum scruglii and Hypericum hircinum
| Autor: | Antonio Fiorentino, Fabiana Antognoni, Monica Scognamiglio, Cinzia Sanna, Ferruccio Poli, Luca Cornioli, Manuela Mandrone, Francesca Bonvicini |
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| Přispěvatelé: | Manuela, Mandrone, Monica, Scognamiglio, Antonio, Fiorentino, Cinzia, Sanna, Luca, Cornioli, Fabiana, Antognoni, Francesca, Bonvicini, Ferruccio, Poli, Mandrone, Manuela, Scognamiglio, Monica, Fiorentino, Antonio, Sanna, Cinzia, Cornioli, Luca, Antognoni, Fabiana, Bonvicini, Francesca, Poli, Ferruccio |
| Jazyk: | angličtina |
| Rok vydání: | 2017 |
| Předmět: |
Antioxidant
Fluoroglucinol medicine.medical_treatment Phytochemicals H NMR profile Decoction Hypericum hircinum Phloroglucinol Biology 01 natural sciences Antioxidants Hypericum hircinum Hypericum perforatum Hypericum scruglii 1H NMR profile Fluoroglucinols α-glucosidase inhibition Hypericum scruglii Chlorocebus aethiops Drug Discovery Hypericum perforatum medicine Maceration (wine) Animals Glycoside Hydrolase Inhibitors Vero Cells IC50 Pharmacology chemistry.chemical_classification Traditional medicine Plant Extracts 010405 organic chemistry α-glucosidase inhibition General Medicine Plant Components Aerial 0104 chemical sciences 010404 medicinal & biomolecular chemistry Enzyme Italy chemistry Phytochemical Quercetin Hypericum |
| Popis: | The phytochemical profile, antioxidant activity and α-glucosidase inhibitory activity of Hypericum scruglii Bacchetta, Brullo et Salmeri and Hypericum hircinum L. were analyzed and compared to those of the best-known Hypericum perforatum L. Samples were prepared using three different extraction methods (maceration in 70% ethanol, extraction in MeOH/phosphate buffer and decoction), the influence of which on the metabolic profile and bioactivities are discussed. H. scruglii and H. hircinum extracted in 70% ethanol strongly inhibited α-glucosidase (IC50 7.25 and 14.05 μg/ml, respectively), which is a valuable enzymatic target for treating metabolic disorders, while H. perforatum was found to be less powerful than the other two species in all the performed biological tests. The phytochemical profile was analyzed by NMR, HPLC-DAD and HPLC-FLD, revealing remarkable differences among the species. In particular, H. scruglii, which is a species endemic to Sardinia Island (Italy), was particularly enriched in two phloroglucinol-derivatives identified by means of 2D NMR and LC/MS/MS experiments as 3-geranyl-1-(2′-methylbutanoyl)-phloroglucinol and 3-geranyl-1-(2′-methylpropanoyl)-phloroglucinol. |
| Databáze: | OpenAIRE |
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