Immunohistochemical localization of the inositol 1,4,5-triphosphate receptor in the human nervous system
| Autor: | Julia M. Polak, A.M. Suburo, José Rodrigo, Ricardo Martínez-Murillo, Katsuhiko Mikoshiba, Marco L. Rossi, G. Terenghi, Nobuaki Maeda |
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| Rok vydání: | 1993 |
| Předmět: |
Male
Nervous system Cerebellum Central nervous system Receptors Cytoplasmic and Nuclear Receptors Cell Surface Biology Mice Purkinje Cells Species Specificity Ganglia Spinal medicine Animals Humans Inositol 1 4 5-Trisphosphate Receptors Rats Wistar Axon Inositol phosphate Molecular Biology Aged Nerve Endings Neurons chemistry.chemical_classification Staining and Labeling General Neuroscience Brain Human brain Middle Aged Inositol trisphosphate receptor Immunohistochemistry Rats medicine.anatomical_structure Spinal Cord nervous system chemistry Female Calcium Channels Neurology (clinical) Neuroscience Immunostaining Brain Stem Developmental Biology |
| Zdroj: | Brain Research. 601:193-202 |
| ISSN: | 0006-8993 |
| Popis: | A monoclonal antibody raised against the mouse cerebellar inositol trisphosphate receptor was used to study the immunohistochemical localization of this protein in the human central nervous system. As in the brain of rodents, strong immunoreactivity was found in dendrites, axon and cell bodies of Purkinje cells, as well as in nerve endings in the cerebellar and vestibular nuclei. Cerebellar efferent fibres were the only positive structures demonstrated in the brainstem and no immunostaining could be detected in the spinal cord or dorsal root ganglia. By contrast, numerous immunoreactive neurons were present in several telencephalic and diencephalic structures, including the brain cortex, hippocampus, basal ganglia, basal forebrain, amygdala and thalamus. Immunostaining of these brain neurons was weaker than that found in Purkinje cells and was evident in cell bodies and dendrites. Thus, the human brain contains a molecule cross-reacting with the mouse inositol trisphosphate receptor protein that is expressed in a pattern similar to that found in rodents. These findings can be of great importance for understanding the function of this protein in normal brain and its modifications in neuropathological disorders. |
| Databáze: | OpenAIRE |
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