Tubular and Spherical SiO2 Obtained by Sol Gel Method for Lipase Immobilization and Enzymatic Activity
| Autor: | Crina Anastasescu, Ioan Balint, Radu Socoteanu, Maria Zaharescu, Mirela Enache, Mihai Anastasescu, Dana C. Culita, Daniel G. Angelescu, Cornel Munteanu, Gabriel Plavan, Adriana Rusu, Jose M. Calderon-Moreno, Mariuca Gartner, Stefan Strungaru, Catalina Gifu, Silviu Preda |
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| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: |
Immobilized enzyme
Silicon dioxide enzymatic catalysis Pharmaceutical Science 02 engineering and technology 010402 general chemistry 01 natural sciences Analytical Chemistry Enzyme catalysis Catalysis lcsh:QD241-441 Hydrolysis chemistry.chemical_compound lcsh:Organic chemistry SiO2 Drug Discovery Physical and Theoretical Chemistry Lipase Sol-gel tubular and spherical morphology biology Chemistry Organic Chemistry 021001 nanoscience & nanotechnology 0104 chemical sciences Isoelectric point Chemical engineering lipase immobilization Chemistry (miscellaneous) biology.protein Molecular Medicine 0210 nano-technology |
| Zdroj: | Molecules, Vol 23, Iss 6, p 1362 (2018) Molecules Volume 23 Issue 6 |
| ISSN: | 1420-3049 |
| Popis: | A wide range of hybrid biomaterials has been designed in order to sustain bioremediation processes by associating sol-gel SiO2 matrices with various biologically active compounds (enzymes, antibodies). SiO2 is a widespread, chemically stable and non-toxic material thus, the immobilization of enzymes on silica may lead to improving the efficiency of biocatalysts in terms of endurance and economic costs. Our present work explores the potential of different hybrid morphologies, based on hollow tubes and solid spheres of amorphous SiO2, for enzyme immobilization and the development of competitive biocatalysts. The synthesis protocol and structural characterization of spherical and tubular SiO2 obtained by the sol gel method were fully investigated in connection with the subsequent immobilization of lipase from Rhizopus orizae. The immobilization is conducted at pH 6, lower than the isoelectric point of lipase and higher than the isoelectric point of silica, which is meant to sustain the physical interactions of the enzyme with the SiO2 matrix. The morphological, textural and surface properties of spherical and tubular SiO2 were investigated by SEM, nitrogen sorption, and electrokinetic potential measurements, while the formation and characterization of hybrid organic-inorganic complexes were studied by UV-VIS, FTIR-ATR and fluorescence spectroscopy. The highest degree of enzyme immobilization (as depicted from total organic carbon) was achieved for tubular morphology and the hydrolysis of p-nitrophenyl acetate was used as an enzymatic model reaction conducted in the presence of hybrid lipase&ndash SiO2 complex. |
| Databáze: | OpenAIRE |
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