Calcium‑dependent activation of PHEX, MEPE and DMP1 in osteocytes

Autor:	Donmez B.O., Karagur E.R., Donmez A.C., Choi J., Akkus O.
Rok vydání:	2022
Předmět:	glycoprotein Cancer Research antioxidant phosphate regulating neutral endopeptidase osteocyte Biochemistry Osteocytes Antioxidants Phosphates reduced nicotinamide adenine dinucleotide phosphate oxidase Genetics oxidative stress oxidizing agent Molecular Biology phosphate Glycoproteins Extracellular Matrix Proteins Caspase 3 Superoxide Dismutase scleroprotein NADPH Oxidases phosphoprotein Catalase Phosphoproteins Oxidants PHEX Phosphate Regulating Neutral Endopeptidase Glutathione bone mineralization proteins Oncology Molecular Medicine MLO-Y4 cell membrane metalloendopeptidase Calcium Neprilysin metabolism
Zdroj:	Molecular medicine reports. 26(6)
ISSN:	1791-3004
Popis:	Calcium (Ca2+) signaling is the first messenger signal exhibited by osteocytes. The present study aimed to better understand the link between Ca2+ concentration, and the levels of bone mineralization regulator proteins [phosphate-regulating neutral endopeptidase on chromo- some X (PHEX), matrix extracellular phosphoglycoprotein (MEPE) and dentin matrix protein 1 (DMP1)] and the levels of oxidative stress in osteocytes. The viability of MLO-Y4 cells was determined using the live/dead assay following treatment with various Ca2+ concentrations (1.8, 6, 12, 18, 24 and 50 mM) for different durations (15 and 60 min, and 24 h). Superoxide dismutase (SOD), catalase (CAT), glutathione (GSH) and NADPH oxidase (NOX) enzymes were analyzed using a colorimetric method. Apoptosis was detected by caspase-3 analysis. Furthermore, the protein expression levels of PHEX, MEPE and DMP1 were analyzed using immunoblotting, and oxidative stress was examined using the total antioxidant and total oxidant status (TOS) assay. Notably, after 15 min, there were more live cells than dead cells; however, after 60 min, the number of dead cells was increased following treatment with 24 and 50 mM Ca2+. After 24 h, there were more dead cells than live cells following treatment with 50 mM Ca2+. After 24 h of Ca2+ treatment, the highest protein expression levels of PHEX, MEPE and DMP1 were measured in cells treated with 24 mM Ca2+. In addition, as Ca2+ concentration increased, the TOS and the oxidative stress index values were also increased. In conclusion, these results suggested that 24 mM Ca2+ may trigger bone mineralization proteins, such as PHEX, MEPE and DMP1, and could be considered an applicable dosage for the treatment of bone damage in the future. © 2022 Spandidos Publications. All rights reserved.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::959c3d17e448b5969b79b1ea4d73de0b https://pubmed.ncbi.nlm.nih.gov/36281920 Zobrazit plný text záznamu Plný text ve formátu PDF Plný text ve formátu HTML
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