Role of vitamin B6 status on antioxidant defenses, glutathione, and related enzyme activities in mice with homocysteine-induced oxidative stress
| Autor: | Chin-Lin Hsu, Wan-Ju Lee, Shih-Chien Huang, Yi-Chia Huang, Chien-Hsiang Cheng, Cheng-Chin Hsu |
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| Přispěvatelé: | This study was supported by the Ministry of Science and Technology, Taiwan (NSC101-2320-B-040-016-MY3) |
| Jazyk: | angličtina |
| Rok vydání: | 2015 |
| Předmět: |
0301 basic medicine
Vitamin medicine.medical_specialty Antioxidant mice Homocysteine Normal diet medicine.medical_treatment 030209 endocrinology & metabolism lcsh:TX341-641 medicine.disease_cause vitamin B6 03 medical and health sciences chemistry.chemical_compound Vitamin B-6 glutathione antioxidant enzyme activities homocysteine-induced oxidative stress 0302 clinical medicine Internal medicine medicine Nutrition chemistry.chemical_classification 030109 nutrition & dietetics Nutrition and Dietetics business.industry Glutathione peroxidase Public Health Environmental and Occupational Health Glutathione Malondialdehyde Endocrinology chemistry Original Article business lcsh:Nutrition. Foods and food supply Oxidative stress Food Science |
| Zdroj: | Food & Nutrition Research, Vol 59, Iss 0, Pp 1-7 (2015) Food & Nutrition Research Food & Nutrition Research; Vol 59 (2015) |
| ISSN: | 1654-661X 1654-6628 |
| Popis: | Background : Vitamin B6 may directly or indirectly play a role in oxidative stress and the antioxidant defense system. Objective : The purpose of this study was to examine the associations of vitamin B6 status with cysteine, glutathione, and its related enzyme activities in mice with homocysteine-induced oxidative stress. Design : Four-week-old male BALB/c mice were weighed and divided into one of four dietary treatment groups fed either a normal diet (as a control group and a homocysteine group), a vitamin B6-deficient diet (as a B6-deficient group), or a B6-supplemented diet (a pyridoxine-HCl-free diet supplemented with 14 mg/kg of pyridoxine-HCl, as a B6 supplement group) for 28 days. Homocysteine thiolactone was then added to drinking water in three groups for 21 days to induce oxidative stress. At the end of the study, mice were sacrificed by decapitation and blood and liver samples were obtained. Results : Mice with vitamin B6-deficient diet had the highest homocysteine concentration in plasma and liver among groups. Significantly increased hepatic malondialdehyde levels were observed in the vitamin B6-deficient group. Among homocysteine-treated groups, mice with vitamin B6-deficient diet had the highest plasma glutathione concentration and relatively lower hepatic glutathione concentration. The glutathione peroxidase activities remained relatively stable in plasma and liver whether vitamin B6 was adequate, deficient, or supplemented. Conclusions : Mice with deficient vitamin B6 intakes had an aggravate effect under homocysteine-induced oxidative stress. The vitamin B6-deficient status seems to mediate the oxidative stress in connection with the redistribution of glutathione from liver to plasma, but not further affect glutathione-related enzyme activities in mice with homocysteine-induced oxidative stress. Keywords : vitamin B6; glutathione; antioxidant enzyme activities; homocysteine-induced oxidative stress; mice (Published: 29 April 2015) Citation: Food & Nutrition Research 2015, 59: 25702 - http://dx.doi.org/10.3402/fnr.v59.25702 |
| Databáze: | OpenAIRE |
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