Changes in submicrometer structure of enzymatically hydrolyzed microcrystalline cellulose
| Autor: | Pentti Jääskeläinen, Matti Siika-aho, Anikó Várnai, Marko Peura, Kirsi Leppänen, Janne Ruokolainen, Liisa Viikari, Jessica Lucenius, Aki Kallonen, Ritva Serimaa, Paavo A. Penttilä |
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| Rok vydání: | 2010 |
| Předmět: |
Materials science
Polymers and Plastics Bioengineering Cellulase Biomaterials Crystallinity chemistry.chemical_compound Microscopy Electron Transmission X-Ray Diffraction Enzymatic hydrolysis Scattering Small Angle Materials Chemistry Cellulases ta318 SDG 7 - Affordable and Clean Energy Cellulose Particle Size ta116 Trichoderma reesei ta515 ta217 ta113 Trichoderma biology ta114 Small-angle X-ray scattering Hydrolysis beta-Glucosidase X-Ray Microtomography biology.organism_classification Microcrystalline cellulose Biochemistry chemistry Chemical engineering Transmission electron microscopy biology.protein Aspergillus niger |
| Zdroj: | Penttilä, P A, Varnai, A, Leppänen, K, Peura, M, Kallonen, A, Jääskeläinen, P, Lucenius, J, Ruokolainen, J, Siika-aho, M, Viikari, L & Serimaa, R 2010, ' Changes in submicrometer structure of enzymatically hydrolyzed microcrystalline cellulose ', Biomacromolecules, vol. 11, no. 4, pp. 1111-1117 . https://doi.org/10.1021/bm1001119 |
| ISSN: | 1526-4602 |
| Popis: | To understand the limitations occurring during enzymatic hydrolysis of cellulosic materials in renewable energy production, we used wide-angle X-ray scattering (WAXS), small-angle X-ray scattering (SAXS), X-ray microtomography, and transmission electron microscopy (TEM) to characterize submicrometer changes in the structure of microcrystalline cellulose (Avicel) digested with the Trichoderma reesei enzyme system. The microtomography measurements showed a clear decrease in particle size in scale of tens of micrometers. In all the TEM pictures, similar elongated and partly ramified structures were observed, independent of the hydrolysis time. The SAXS results of rewetted samples suggested a slight change in the structure in scale of 10−20 nm, whereas the WAXS results confirmed that the degree of crystallinity and the crystal sizes remained unchanged. This indicates that the enzymes act on the surface of cellulose bundles and are unable to penetrate into the nanopores of wet cellulose. |
| Databáze: | OpenAIRE |
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