Recombinant Retroviral Vector Delivered Intramuscularly Localizes to the Site of Injection in Mice
Autor: | Nancy Sajjadi, Tammi Howard, Wilson Edwards, Doug J. Jolly, Edgar Kamantigue, Steven Mento, Sunil Chada |
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Rok vydání: | 1994 |
Předmět: |
Male
viruses Genetic Vectors Molecular Sequence Data DNA Recombinant Thymus Gland Kidney Injections Intramuscular Polymerase Chain Reaction law.invention Viral vector Mice Transduction (genetics) Retrovirus Proviruses law Testis Murine leukemia virus Genetics Animals Cytotoxic T cell Molecular Biology Mice Inbred BALB C Base Sequence biology Kidney metabolism biology.organism_classification Virology Molecular biology Liver DNA Viral HIV-1 Recombinant DNA Molecular Medicine Spleen Ex vivo |
Zdroj: | Human Gene Therapy. 5:693-699 |
ISSN: | 1557-7422 1043-0342 |
Popis: | A murine retroviral vector encoding the human immunodeficiency virus type 1 (HIV-1) env and rev genes can be used to induce cytotoxic T lymphocyte responses. Immune responses can be induced by an ex vivo treatment, in which autologous cells are transduced in vitro and re-introduced to the donor, or by direct administration of retroviral vector via intramuscular injection. In this study we have used polymerase chain reaction (PCR) analysis to examine the distribution of recombinant murine retrovirus directly administered to mice. Mice were injected intramuscularly with HIV-IT(V), an amphotropic murine leukemia virus (MLV)-based retroviral vector carrying the HIV-1 env/rev genes and a neomycin resistance marker gene. Detection of the HIV-1 env gene in DNA isolated from injection sites demonstrated in vivo transduction. No evidence of transduction was observed in the testes, spleen, kidney, or thymus. Retroviral DNA was detected in the liver of one animal in the study. These data suggest that retroviral vector administered intramuscularly to mice localizes primarily to the site of injection and that measurable transduction in the testes does not occur. |
Databáze: | OpenAIRE |
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