Serological cross-reactivity between a human Ro/SS-A autoantigen (calreticulin) and the lambda Ral-1 antigen of Onchocerca volvulus
| Autor: | R Lucius, J D Capra, F A Lux, D W Büttner, Daniel P. McCauliffe, Richard D. Sontheimer, Tsu-San Lieu |
|---|---|
| Rok vydání: | 1992 |
| Předmět: |
medicine.drug_class
Immunoblotting Molecular Sequence Data Cross Reactions Onchocerciasis medicine.disease_cause Monoclonal antibody Autoantigens Cross-reactivity Serology Antigen RNA Small Cytoplasmic medicine Animals Humans Amino Acid Sequence Onchocerca Cell Line Transformed biology Calcium-Binding Proteins Antibodies Monoclonal Helminth Proteins General Medicine Blotting Northern biology.organism_classification Precipitin Tests Onchocerca volvulus Virology Molecular biology Ribonucleoproteins Antigens Helminth biology.protein Antibody Calreticulin Research Article |
| Zdroj: | Journal of Clinical Investigation. 89:1945-1951 |
| ISSN: | 0021-9738 |
| DOI: | 10.1172/jci115801 |
| Popis: | We have cloned and sequenced a 46-kD Ro/SS-A autoantigen gene that is the human homologue of the calcium-binding protein, calreticulin. The sequence of this 46-kD Ro/SS-A protein (calreticulin) has significant homology to lambda Ral-1, a recombinant cDNA clone corresponding to a major antigen of the nematode, Onchocerca volvulus, the infectious agent of onchocerciasis. We therefore sought to determine whether antibodies produced by onchocerciasis patients might crossreact with the human 46-kD Ro/SS-A autoantigen (calreticulin). 20 of 22 sera from Liberian onchocerciasis patients who had no known evidence of autoimmune disease were found to contain antibodies that reacted with the 46-kD Ro/SS-A (calreticulin) by immunoblot analysis. Characteristic of sera reactive with Ro/SS-A antigens, some onchocerciasis sera also immunoprecipitated the Ro/SS-A-associated hY RNAs. In addition, a monoclonal antibody raised against O. volvulus organisms reacted to purified human WiL-2 cell 46 kD Ro/SS-A antigen (calreticulin) by ELISA. These results strongly suggest that onchocerciasis patients produce antibodies that crossreact with the 46-kD human Ro/SS-A autoantigen (calreticulin) and raise the possibility that infectious organisms such as O. volvulus might play a triggering or exacerbating role in the human Ro/SS-A autoimmune response. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|