Histological study on the protective effect of endogenous stem-cell mobilization in Adriamycin-induced chronic nephropathy in rats
| Autor: | Dalia Ibrahim Ismail, Eman Mostafa Sadek, Nagla M. Salama, Asmaa Ahmed El-Shafei |
|---|---|
| Jazyk: | angličtina |
| Předmět: |
0301 basic medicine
medicine.medical_specialty caspase-3 adriamycin Urology H&E stain Pathology and Forensic Medicine Nephropathy 03 medical and health sciences chemistry.chemical_compound Trichrome Medicine Instrumentation Blood urea nitrogen Creatinine Kidney business.industry medicine.disease Electronic Optical and Magnetic Materials Granulocyte colony-stimulating factor Surgery 030104 developmental biology medicine.anatomical_structure chemistry Nephrology Original Article CD34 granulocyte colony-stimulating factor business chronic kidney disease Kidney disease |
| Zdroj: | Journal of Microscopy and Ultrastructure |
| ISSN: | 2213-879X |
| DOI: | 10.1016/j.jmau.2015.12.003 |
| Popis: | Chronic kidney disease is a global health problem with increasing morbidity and mortality. Therefore, this study was planned to test the protective effect of hematopoietic-stem-cell mobilization by granulocyte colony-stimulating factor (G-CSF) on Adriamycin (ADR)-induced chronic renal disease in rats. Thirty albino rats were equally divided into three groups: control, ADR group [rats received a single intravenous injection of ADR (5mg/kg)], and G-CSF group [rats received ADR by the same route and the same dose as the previous group, and then G-CSF (70μg/kg/d) 2 hours after ADR injection then daily for five consecutive days]. At the time of sacrifice (after 6 weeks), blood samples were taken to estimate the blood urea nitrogen and serum creatinine. Kidney sections were stained with hematoxylin and eosin, toluidine blue, Masson's trichrome, periodic acid–Schiff stains, and immunohistochemical staining against CD34 and caspase-3. The G-CSF group exhibited protection against renal injury manifested by reducing blood urea nitrogen and serum-creatinine levels, improving histological architecture, and increasing the proliferative capacity of renal tubules. |
| Databáze: | OpenAIRE |
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