Development of a pyrosequencing assay for rapid assessment of quinolone resistance in Acinetobacter baumannii isolates

Autor:	Angelina Trifonova, Mihaela Ariciuc, Leonid M. Irenge, Yann Deccache, Alexandra Macovei, Jérôme Ambroise, Encho Savov, Raymond Vanhoof, Jean-Luc Gala, Ivanka Gergova
Rok vydání:	2010
Předmět:	Microbiology (medical) Acinetobacter baumannii DNA Topoisomerase IV Molecular Sequence Data Drug resistance Microbial Sensitivity Tests Microbiology Quinolone resistance Bacterial Proteins Drug Resistance Bacterial Humans heterocyclic compounds Molecular Biology Gene Genetics biology Base Sequence Sequence Analysis DNA biochemical phenomena metabolism and nutrition bacterial infections and mycoses biology.organism_classification Rapid assessment DNA Gyrase Mutation Quinolines bacteria Pyrosequencing Acinetobacter Infections
Zdroj:	Journal of microbiological methods. 86(1)
ISSN:	1872-8359
Popis:	Rapid and reliable assessment of Acinetobacter baumannii resistance to quinolones was successfully achieved through pyrosequencing of the gyrA and parC quinolone-resistance determining regions. A strong correlation was found between quinolone resistance and mutations in gyrA codon 83 and/or in the parC gene (codons 80 or 84). Absence of QRDR mutations was associated with susceptibility to quinolones.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::95386ac69fe226d99ff8db819d217fd9 https://pubmed.ncbi.nlm.nih.gov/21514328 Zobrazit plný text záznamu Full Text from ScienceDirect