Effects of Scutellaria baicalensis Georgi on Macrophage-Hepatocyte Interaction Through Cytokines Related to Growth Control of Murine Hepatocytes
Autor: | Jen-Hwey Chiu, Wing Yiu Lui, Chin-Wen Chi, Ann-Ping Tsou, Jir You Wang, Shu Ling Fu, Tung Hu Tsai, Chew Wun Wu, Chen-Kung Chou, Han Ning Chuang, Cheng Po Hu, Sheau Farn Yeh |
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Rok vydání: | 2006 |
Předmět: |
Male
0301 basic medicine Kupffer Cells medicine.medical_treatment Cell Count Cell Communication Biology General Biochemistry Genetics and Molecular Biology Cell Line Transforming Growth Factor beta1 Mice 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Transforming Growth Factor beta medicine Animals Cells Cultured Cell Proliferation Mice Inbred BALB C Interleukin-6 Plant Extracts Tumor Necrosis Factor-alpha Kupffer cell biology.organism_classification Molecular biology Liver regeneration 030104 developmental biology medicine.anatomical_structure Cytokine Bromodeoxyuridine chemistry Cell culture 030220 oncology & carcinogenesis Hepatocyte Hepatocytes Scutellaria baicalensis Trypan blue Transforming growth factor |
Zdroj: | Experimental Biology and Medicine. 231:444-455 |
ISSN: | 1535-3699 1535-3702 |
Popis: | The aim of this study is to elucidate the effects of Scutellaria baicalensis Georgi (SbG) extract and its constituents on macrophage-hepatocyte interaction in primary cultures. By using trans-well primary Kupffer cell culture or conditioned medium (CM) from murine macrophage RAW264.7 cell line (RAW cells), effects of SbG on hepatocyte growth were evaluated by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-tetrazolium bromide and trypan blue exclusion assay. Cytokine production, antibody-neutralization studies, and molecular mechanisms of transforming growth factor (TGF)-beta1 gene expression were elucidated on SbG-treated RAW264.7 cells. In addition, recombinant human TGF-beta1 (r-human TGF-beta1) was added to elucidate the mechanisms of SbG effects on cultured hepatocytes. Immunohistochemistry using anti-NF-kappaB antibody was used to determine the possible signal transduction pathways in primary hepatocyte culture. The results showed that SbG stimulated the proliferation of cultured hepatocytes, possibly through NF-kappaB, but not of Toll-like receptor 4 activation; whereas SbG-RAW-CM and SbG in trans-well significantly suppressed the proliferation of hepatocytes. Antibody-neutralization studies revealed that TGF-beta1 was the main antimitotic cytokine in SbG-treated RAW cells CM. The growth stimulation effect of SbG on cultured hepatocytes was inhibited by exogenous administration of r-human TGF-beta1. Furthermore, SbG induced NF-kB translocation into the nuclei of cultured cells. In the RAW264.7 line, SbG and baicalin stimulated TGF-beta1 gene expression via NF-kappaB and protein kinase C activation. We conclude that SbG stimulates hepatocyte growth via activation of the NF-kappaB pathway and induces TGF-beta1 gene expression through the Kupffer cell-hepatocyte interaction, which subsequently results in the inhibition of SbG-stimulated hepatocyte growth. |
Databáze: | OpenAIRE |
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