Sea urchin embryos as a model system for studying autophagy induced by cadmium stress
| Autor: | Roberto Chiarelli, Maria Agnello, Maria Carmela Roccheri |
|---|---|
| Přispěvatelé: | Chiarelli, R, Agnello, M, Roccheri, MC |
| Rok vydání: | 2011 |
| Předmět: |
Embryo
Nonmammalian animal structures Immunoblotting Fluorescent Antibody Technique chemistry.chemical_element Biology Models Biological Paracentrotus lividus Stress Physiological Heat shock protein Botany Autophagy Animals Settore BIO/06 - Anatomia Comparata E Citologia autophagy cadmium stress acidic vesicular organelles bafilomycin A1 LC3 Paracentrotus lividus embryos Molecular Biology Organelles Cadmium Staining and Labeling Embryogenesis Embryo Cell Biology biology.organism_classification Embryonic stem cell Acridine Orange Cell biology chemistry Neutral Red Apoptosis embryonic structures Paracentrotus Microtubule-Associated Proteins Densitometry |
| Zdroj: | Autophagy. 7:1028-1034 |
| ISSN: | 1554-8635 1554-8627 |
| DOI: | 10.4161/auto.7.9.16450 |
| Popis: | It is well known that sea urchin embryos are able to activate different defense strategies against stress. We previously demonstrated that cadmium treatment triggers the accumulation of metal in embryonic cells and the activation of defense systems depending on concentration and exposure time, through the synthesis of heat shock proteins and/or the initiation of apoptosis. Here we show that Paracentrotus lividus embryos exposed to Cd adopt autophagy as an additional stratagem to safeguard the developmental program. At present, there are no data focusing on the role of this process in embryo development of marine organisms. In this paper we utilized different techniques to detect autophagy in sea urchin embryos. Using neutral red (NR) and acridine orange (AO) vital staining, we found that embryos exposed to Cd display massive punctiform spots in the cytoplasm, indicative of acidic vesicular organelles (AVOs). These results have been confirmed using bafilomycin A1, a known inhibitor of this process. Furthermore these data were validated through both protein gel blotting and immunofluorescence in situ analysis of LC3-II, a specific marker of autophagy. We found that in P. lividus embryos autophagic processes occur in lesser amounts during physiological development and in greater amounts after Cd exposure |
| Databáze: | OpenAIRE |
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