Transcriptomically-guided mesendoderm induction of human pluripotent stem cells using a systematically defined culture scheme
| Autor: | Chandarong Choey, Julien Yockell-Lelièvre, Sarah Y. Kwon, Mirabelle Ho, William L. Stanford, R Matthew Tanner, Richard L. Carpenedo, Theodore J. Perkins, Carole Doré, Duncan J. Stewart |
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| Rok vydání: | 2019 |
| Předmět: |
0303 health sciences
Mesoderm animal structures Cell Ectoderm formation Biology Chondrogenesis Endothelial progenitor cell Cell biology 03 medical and health sciences 0302 clinical medicine medicine.anatomical_structure Tissue engineering embryonic structures medicine Endoderm Induced pluripotent stem cell 030217 neurology & neurosurgery 030304 developmental biology |
| Popis: | SummaryHuman pluripotent stem cells (hPSCs) are an essential cell source in tissue engineering, studies of development, and disease modeling. Efficient, broadly amenable protocols for rapid lineage induction of hPSCs are of great interest in the stem cell biology field. We describe a simple, robust method for differentiation of hPSCs into mesendoderm in defined conditions utilizing single-cell seeding (SCS) and BMP4 and Activin A (BA) treatment. Gene sets and gene ontology terms related to mesoderm and endoderm differentiation were enriched after 48 hours of BA treatment. BA treatment was readily incorporated into existing protocols for chondrogenic and endothelial progenitor cell differentiation. After prolonged differentiation in vitro or in vivo, BA pre-treatment resulted in higher mesoderm and endoderm levels at the expense of ectoderm formation. These data demonstrate that SCS with BA treatment is a powerful method for induction of mesendoderm that can be integrated into protocols for mesoderm and endoderm differentiation. |
| Databáze: | OpenAIRE |
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