Monitoring receptor oligomerization by line-scan fluorescence cross-correlation spectroscopy

Autor:	Hink, M.A., Postma, M., Conn, P.M.
Přispěvatelé:	Molecular Cytology (SILS, FNWI)
Jazyk:	angličtina
Rok vydání:	2013
Předmět:	HeLa biology Biochemistry Diffusion Fluorescence cross-correlation spectroscopy Spectroscopy biology.organism_classification Line scan Receptor Fluorescence
Zdroj:	Receptor-receptor interactions, 197-212 STARTPAGE=197;ENDPAGE=212;TITLE=Receptor-receptor interactions
ISSN:	0091-679X
DOI:	10.1016/b978-0-12-408143-7.00011-6
Popis:	Membrane-localized receptor proteins are involved in many signaling cascades, and diffusion and oligomerization are key processes controlling their activity. In order to study these processes in living cells, fluorescence fluctuation spectroscopy techniques have been developed that allow the quantification of concentration levels, diffusion rates, and interactions between fluorescently labeled receptor proteins at the nanomolar concentration level. This chapter presents a brief introduction to the technique and a protocol to measure and quantify the diffusion and oligomerization of human histamine1 receptor complexes in living HeLa cells using line-scanning fluorescence cross-correlation spectroscopy.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::94ac203ed620fd1a98e79b6071f17299 https://doi.org/10.1016/b978-0-12-408143-7.00011-6 Zobrazit plný text záznamu