Hepatic tumor spread of colorectal cancer in a laparoscopic animal model
| Autor: | V. Paolucci, M. Lorenz, Z. G. Kim, T. Gessmann, C. N. Gutt |
|---|---|
| Rok vydání: | 2000 |
| Předmět: |
Male
medicine.medical_specialty Colorectal cancer Rectum Adenocarcinoma Gastroenterology Metastasis Liver Neoplasms Experimental Internal medicine Medicine Animals Laparoscopy medicine.diagnostic_test business.industry Rats Inbred Strains Hepatology medicine.disease Colorectal surgery Rats Disease Models Animal medicine.anatomical_structure Surgery business Complication Colorectal Neoplasms Neoplasm Transplantation Abdominal surgery |
| Zdroj: | Surgical endoscopy. 14(5) |
| ISSN: | 0930-2794 |
| Popis: | We devised a standardized animal model to study the impact of laparoscopic colorectal surgery on intrahepatic tumor cell growth.The technique of laparoscopic surgery in the rat was extended by endoscopic inoculation of colon cancer cells (CC531) into the portal vein (1 x 10(4), 5 x 10(4), 1 x 10(5), 3 x 10(5), 5 x 10(5) cells/ml) of WAG/Rij rats (n = 25). As controls, five animals underwent laparotomy and open intraportal inoculation of 5 x 10(4) cells/ml.Hepatic tumor growth occurred after inoculation of 5 x 10(4), 1 x 10(5), 3 x 10(5), and 5 x 10(5) cells/ml. Extrahepatic tumor and conflating hepatic tumor was observed after the inoculation of 1 x 10(5), 3 x 10(5), and 5 x 10(5) cells/ml. Concentrations of 5 x 10(4) cells/ml injected either laparoscopically or via an open technique led to single hepatic tumor nodules. No tumor growth was seen after inoculation of 1 x 10(4) cells/ml.Laparoscopic intraportal tumor cell inoculation is a feasible technique to create hepatic metastases. The inoculation of 5 x 10(4) CC531 cells leads to reliable cell growth that can be used to investigate the impact of various laparoscopic techniques on tumor spread. |
| Databáze: | OpenAIRE |
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