Concanavalin A-mediated T cell proliferation is regulated by herpes virus entry mediator costimulatory molecule
| Autor: | Chika Yasuoka, Manabu Inobe, Yoshiaki Ando, Takuya Mishima, Tsukasa Matsunaga, Toshimitsu Uede, Takuya Ikematsu |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2014 |
| Předmět: |
T-Lymphocytes
T cell Herpesvirus 1 Cercopithecine chemical and pharmacologic phenomena Ig-fusion molecule Biology Lymphocyte Activation chemistry.chemical_compound CD28 Antigens Concanavalin A medicine Extracellular Humans Receptor Costimulatory signal Cell Proliferation T cell activation CD28 Cell Biology General Medicine Immunoglobulin Fc Fragments Cell biology medicine.anatomical_structure chemistry Polyclonal antibodies Immunoglobulin G Mitogen-activated protein kinase Ionomycin biology.protein Receptors Tumor Necrosis Factor Member 14 Lectin Signal Transduction Developmental Biology |
| Zdroj: | In Vitro Cellular and Developmental Biology - Animal. 50(4):313-320 |
| ISSN: | 1071-2690 |
| Popis: | T cell activation is regulated by two distinct signals, signals one and two. Concanavalin A (ConA) is an antigen-independent mitogen and functions as signal one inducer, leading T cells to polyclonal proliferation. CD28 is known to be one of major costimulatory receptors and to provide signal two in the ConA-induced T cell proliferation. Here, we have studied the implication of other costimulatory pathways in the ConA-mediated T cell proliferation by using soluble recombinant proteins consisting of an extracellular domain of costimulatory receptors and Fc portion of human IgG. We found that T cell proliferation induced by ConA, but not PMA plus ionomycin or anti-CD3 mAb, is significantly inhibited by herpes virus entry mediator (HVEM)-Ig, even in the presence of CD28 signaling. Moreover, the high concentration of HVEM-Ig molecules almost completely suppressed ConA-mediated T cell proliferation. These results suggest that HVEM might play more important roles than CD28 in ConA-mediated T cell proliferation. © 2013 The Society for In Vitro Biology. |
| Databáze: | OpenAIRE |
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