Booster-Dependent Alterations of the Subsets of T Lymphocytes and Eosinophils in the Bronchi of Immunized Guinea Pigs

Autor:	B. Boris Vargaftig, David Baker, J.R. Lapa e Silva, Claude-Marie Bachelet, R. J. Scheper, Marina Pretolani
Rok vydání:	1992
Předmět:	Bronchus Lung biology Immunology General Medicine T lymphocyte respiratory system Eosinophil Guinea pig Ovalbumin medicine.anatomical_structure Eosinophil migration medicine biology.protein Immunology and Allergy sense organs CD8
Zdroj:	International Archives of Allergy and Immunology. 99:350-353
ISSN:	1423-0097 1018-2438
DOI:	10.1159/000236282
Popis:	We investigated whether guinea pigs sensitized to ovalbumin show changes in T cells in the bronchial wall and whether they correlate with eosinophil migration. Animals received two injections of 10 µg ovalbumin in Al(OH)3 with a 2-week interval. Lungs were resected and frozen and cryostat sections stained with monoclonal antibodies recognizing T cells and subsets. Eosinophil peroxidase staining was used to identify this cell type. Sections stained with each marker were read 'blind' and cells enumerated in the bronchial lamina propria. A large number of T cells, mainly of CD4+ subset, were recruited into the bronchi 7 days after the booster injection of the antigen, in contrast to nonimmunized or nonboosted animals. These changes were also accompanied by an infiltration of eosinophils in boosted animals and suggest that T cells and/or their products play an important role in the development of bronchial hyperreactivity in this model.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::945bac5fb8d5c3ae9f413437dffbabe8 https://doi.org/10.1159/000236282 Zobrazit plný text záznamu