Cell surface processing of the P1 adhesin of Mycoplasma pneumoniae identifies novel domains that bind host molecules
| Autor: | Steven P. Djordjevic, Veronica M. Jarocki, Michael Widjaja, Iain J. Berry, Roger Dumke, Matthew P. Padula |
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| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
Mycoplasma pneumoniae 030106 microbiology lcsh:Medicine Proteomic analysis Cleavage (embryo) medicine.disease_cause Article Bacterial Adhesion 03 medical and health sciences Mycoplasma hyopneumoniae medicine Humans lcsh:Science Adhesins Bacterial Cytoskeleton Multidisciplinary biology Chemistry lcsh:R Blood Proteins Bacterial pathogenesis biology.organism_classification Cell biology Attachment organelle Bacterial adhesin Cytoskeletal Proteins 030104 developmental biology Ectodomain A549 Cells Proteolysis Host-Pathogen Interactions Proteome lcsh:Q Pathogens Protein Processing Post-Translational Protein Binding |
| Zdroj: | Scientific Reports Scientific Reports, Vol 10, Iss 1, Pp 1-16 (2020) |
| ISSN: | 2045-2322 |
| DOI: | 10.1038/s41598-020-63136-y |
| Popis: | Mycoplasma pneumoniae is a genome reduced pathogen and causative agent of community acquired pneumonia. The major cellular adhesin, P1, localises to the tip of the attachment organelle forming a complex with P40 and P90, two cleavage fragments derived by processing Mpn142, and other molecules with adhesive and mobility functions. LC-MS/MS analysis of M. pneumoniae M129 proteins derived from whole cell lysates and eluents from affinity matrices coupled with chemically diverse host molecules identified 22 proteoforms of P1. Terminomics was used to characterise 17 cleavage events many of which were independently verified by the identification of semi-tryptic peptides in our proteome studies and by immunoblotting. One cleavage event released 1597TSAAKPGAPRPPVPPKPGAPKPPVQPPKKPA1627 from the C-terminus of P1 and this peptide was shown to bind to a range of host molecules. A smaller synthetic peptide comprising the C-terminal 15 amino acids, 1613PGAPKPPVQPPKKPA1627, selectively bound cytoskeletal intermediate filament proteins cytokeratin 7, cytokeratin 8, cytokeratin 18, and vimentin from a native A549 cell lysate. Collectively, our data suggests that ectodomain shedding occurs on the surface of M. pneumoniae where it may alter the functional diversity of P1, Mpn142 and other surface proteins such as elongation factor Tu via a mechanism similar to that described in Mycoplasma hyopneumoniae. |
| Databáze: | OpenAIRE |
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