Acoustic droplet vaporization-mediated dissolved oxygen scavenging in blood-mimicking fluids, plasma, and blood
| Autor: | Kevin J. Haworth, Raj M. Manglik, Deepak Saagar Kalaikadal, Andrew N. Redington, Christy K. Holland, John N. Lorenz, Karla P. Mercado-Shekhar, Haili Su |
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| Rok vydání: | 2018 |
| Předmět: |
endocrine system
Acoustics and Ultrasonics Diffusion Mixing (process engineering) 02 engineering and technology 010402 general chemistry 01 natural sciences Article Inorganic Chemistry Surface tension Viscosity Plasma Biomimetic Materials medicine Chemical Engineering (miscellaneous) Environmental Chemistry Surface Tension Radiology Nuclear Medicine and imaging Whole blood Acoustic droplet vaporization Chromatography Polyvinylpyrrolidone Chemistry Phantoms Imaging Organic Chemistry Partial pressure Acoustics 021001 nanoscience & nanotechnology 0104 chemical sciences Oxygen Volatilization 0210 nano-technology medicine.drug |
| Zdroj: | Ultrason Sonochem |
| ISSN: | 1873-2828 |
| Popis: | Acoustic droplet vaporization (ADV) has been shown to reduce the partial pressure of oxygen (PO(2)) in a fluid. The goals of this study were three-fold: 1) to determine the ADV pressure amplitude threshold in fluids that had physiologically relevant values for surface tension, protein concentration, and viscosity; 2) to assess whether these parameters and fluid mixing affect ADV-mediated PO(2) reduction; and 3) to assess the feasibility of ADV-mediated PO(2) reduction in plasma and whole blood. In vitro ADV experiments were conducted using perfluoropentane droplets (number density: 5×l0(6)±0.2×l0(6)/mL) dispersed in fluids (saline, polyvinylpyrrolidone solutions, porcine plasma, or porcine whole blood) that had a physiological range of surface tensions (62–68 mN/m), protein concentrations (0 and 68.7 mg/mL), and viscosities (0.7–4 cP). Droplets were exposed to pulsed ultrasound (5 MHz, 4.25 MPa peak negative pressure) while passing through a 37 °C flow system with inline PO(2) sensors. In select experiments, the fluid also passed through mixing channels after ultrasound exposure. Our results revealed that the ADV pressure thresholds were the same for all fluids. Surface tension and protein concentration had no effect on PO(2) reduction. Increasing viscosity attenuated PO(2) reduction. However, the attenuated effect was absent after fluid mixing. Furthermore, ADV-mediated PO(2) reduction in whole blood (30.8 ± 3.2 mmHg) was less than that in a polyvinylpyrrolidone solution (40.2 ± 2.1 mmHg) with equal viscosity. These findings should be considered when planning clinical studies of ADV-mediated PO(2) reduction and other biomedical applications of ADV. |
| Databáze: | OpenAIRE |
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