Kinetic evidence for calcium-ion and phosphate-ion transport systems in mitochondria from ehrlich ascites tumour cells
| Autor: | Rupert F. W. Thorne, Fyfe L. Bygrave |
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| Rok vydání: | 1975 |
| Předmět: |
Anions
Time Factors Biophysics Biological Transport Active chemistry.chemical_element Mitochondria Liver Oxidative phosphorylation Calcium Mitochondrion Biology Ehrlich ascites Biochemistry Phosphate ion transport Phosphates Mice Lanthanum Structural Biology Ascites Genetics medicine Animals Neoplasm Carcinoma Ehrlich Tumor Molecular Biology Cell Biology Metabolism medicine.disease Ruthenium Red Mitochondria Kinetics chemistry medicine.symptom |
| Zdroj: | FEBS Letters. 56:185-188 |
| ISSN: | 0014-5793 |
| DOI: | 10.1016/0014-5793(75)81087-8 |
| Popis: | Calcium ions accumulated by tightly-coupled mitochondria from Ehrlich ascites tumour cells, fail to uncouple oxidative phosphorylation [l] . As a consequence, a number of energy-linked reactions in these mitochondria also are unaffected by high concentrations of Ca2+ [2-41. Especially significant is the finding that aspects of this resistance to Ca2+ are seen as well in mitochondria isolated from several ascitic and solid tumours but not in those from rapidlydividing cells of regenerating rat liver [5] . The purpose of the present study was two-fold; first to establish by a kinetic analysis [6] whether a Ca2’ transport system exists in the ascites tumour mitochondria similar to that in mitochondria from normal mammalian cells (cf. [7]) and second, to determine if any properties of this transport system might be sufficiently abnormal as to contribute to the observed resistance of the tumour mitochondria to the accumulated Ca’+. |
| Databáze: | OpenAIRE |
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