Marker-Assisted Selection for Recognizing Wheat Mutant Genotypes Carrying HMW Glutenin Alleles Related to Baking Quality
| Autor: | Marzieh Varasteh Shamsi, Mohammad Javad Zamani, Mohammad Reza Bihamta, Mohammad Taher Hallajian, Behnam Naserian Khiabani, Zahra Tahernezhad |
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| Rok vydání: | 2014 |
| Předmět: |
Genetic Markers
Genotype Glutens Article Subject Mutant lcsh:Medicine Polymerase Chain Reaction lcsh:Technology General Biochemistry Genetics and Molecular Biology law.invention Glutenin law Storage protein Cooking Allele lcsh:Science Alleles Triticum Polymerase chain reaction General Environmental Science Genetics chemistry.chemical_classification biology lcsh:T lcsh:R food and beverages Bread General Medicine Marker-assisted selection chemistry Genetic marker Mutation biology.protein Electrophoresis Polyacrylamide Gel lcsh:Q Research Article |
| Zdroj: | The Scientific World Journal, Vol 2014 (2014) The Scientific World Journal |
| ISSN: | 1537-744X 2356-6140 |
| DOI: | 10.1155/2014/387912 |
| Popis: | Allelic diversity of HMW glutenin loci in several studies revealed that allelic combinations affect dough quality. Dx5 + Dy10 subunits are related to good baking quality and Dx2 + Dy12 are related to undesirable baking quality. One of the most regular methods to evaluate the baking quality is SDS-PAGE which is used to improve baking quality labs. Marker-assisted selection is the method which can recognize the alleles related to baking quality and this method is based on polymerase chain reaction. 10 pairs of specific primers related to Dx2, Dx2.1, Dx5, Dy10, and Dy12 subunits were used for recognizing baking quality of some wheat varieties and some mutant genotypes. Only 5 pairs of them could show the specific bands. All subunits were recognized by the primers except Dx2.1. Some of the primers were extracted from previous studies and the others were designed based on D genome subunits of wheat. SDS-PAGE method accomplished having confidence in these marker’s results. To realize the effect of mutation, seed storage proteins were measured. It showed that mutation had effect on the amount of seed storage protein on the mutant seeds (which showed polymorphism). |
| Databáze: | OpenAIRE |
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