Design, synthesis, DNA-binding affinity, cytotoxicity, apoptosis, and cell cycle arrest of Ru(II) polypyridyl complexes
| Autor: | K Nagasuryaprasad, Mallepally Rajender Reddy, N. Deepika, Putta Venkat Reddy, Chintakuntla Nagamani, Srishailam Avudoddi, Surya S. Singh, Yata Praveen Kumar, Sirasani Satyanarayana |
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| Rok vydání: | 2015 |
| Předmět: |
Staphylococcus aureus
Stereochemistry Pyridines Phenanthroline Biophysics chemistry.chemical_element Apoptosis Biochemistry Ruthenium HeLa chemistry.chemical_compound Bipyridine Escherichia coli Humans MTT assay Cytotoxicity Molecular Biology biology Ligand Cytotoxins Active site Cell Biology Cell Cycle Checkpoints biology.organism_classification chemistry biology.protein HeLa Cells |
| Zdroj: | Analytical biochemistry. 485 |
| ISSN: | 1096-0309 |
| Popis: | A novel polypyridyl ligand CNPFIP (CNPFIP=2-(5(4-chloro-2-nitrophenyl)furan-2-yl)-1H-imidazo[4,5f][1,10]phenanthroline) and its mononuclear Ru(II) polypyridyl complexes of [Ru(phen)2CNPFIP](2+)(1) (phen=1,10-phenanthroline), [Ru(bpy)2CNPFIP](2+)(2) (bpy=2,2'-bipyridine), and [Ru(dmb)2CNPFIP](2+)(3) (dmb=4,4'-dimethyl-2,2'-bipyridine) have been synthesized successfully and characterized thoroughly by elemental analysis, UV/Vis, IR, NMR, and ESI-MS. The interaction of the Ru(II) complexes with calf thymus DNA (CT-DNA) was investigated by absorption titration, fluorescence, viscosity measurements. The experimental results suggest that three complexes bind to CT-DNA through an intercalative mode and the DNA-binding affinity of complex 1 is greater than that of complexes 2 and 3. The photocleavage of plasmid pBR322 DNA by ruthenium complexes 1, 2, and 3 was investigated. We have also tested three complexes for their antimicrobial activity against Escherichia coli (Gram-negative) and Staphylococcus aureus (Gram-positive) bacteria. The in vitro cytotoxicity of these complexes was evaluated by MTT assay, and complex 1 shows higher cytotoxicity than 2 and 3 on HeLa cells. The induced apoptosis and cell cycle arrest of HeLa cells were investigated by flow cytometry for 24h. The molecular docking of ruthenium complexes 1, 2, and 3 with the active site pocket residues of human DNA TOP1 was performed using LibDock. |
| Databáze: | OpenAIRE |
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