Failure of immunocompetitive capillary electrophoresis assay to detect disease-specific prion protein in buffy coat from humans and chimpanzees with Creutzfeldt-Jakob disease
| Autor: | Heino Diringer, Paul Brown, Oksana Yakovleva, Evgueni L. Saenko, Serguei Soukharev, Larisa Cervenakova, William N. Drohan |
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| Rok vydání: | 2003 |
| Předmět: |
Disease specific
Pan troglodytes PrPSc Proteins animal diseases Bovine spongiform encephalopathy Molecular Sequence Data Clinical Biochemistry Buffy coat Disease Biology Biochemistry Antibodies Creutzfeldt-Jakob Syndrome Analytical Chemistry law.invention Capillary electrophoresis law mental disorders Leukocytes medicine Animals Humans Amino Acid Sequence Prion protein Transmissible spongiform encephalopathy Electrophoresis Capillary medicine.disease Virology nervous system diseases Evaluation Studies as Topic Mutation Recombinant DNA Binding Sites Antibody Peptides |
| Zdroj: | ELECTROPHORESIS. 24:853-859 |
| ISSN: | 1522-2683 0173-0835 |
| DOI: | 10.1002/elps.200390107 |
| Popis: | The emergence of a new environmentally caused variant of Creutzfeldt-Jakob disease (vCJD), the result of food-born infection by the causative agent of bovine spongiform encephalopathy (BSE), has stimulated research on a practical diagnostic screening test. The immunocompetitive capillary electrophoresis (ICCE) assay has been reported to detect disease-specific, proteinase-resistant prion protein (PrPres) in the blood of scrapie-infected sheep. We have applied this method to blood from CJD-infected chimpanzees and humans. The threshold of detection achieved with our ICCE was 0.6 nM of synthetic peptide corresponding to the prion protein (PrP) C-terminus, and 2 nM of recombinant human PrP at the optimized conditions. However, the test was unable to distinguish between extracts of leucocytes from healthy and CJD-infected chimpanzees, and from healthy human donors and patients affected with various forms of CJD. Thus, the ICCE assay as presently performed is not suitable for use as a screening test in human transmissible spongiform encephalopathies (TSEs). |
| Databáze: | OpenAIRE |
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