Construction and Analysis of a Modified Tn4001Conferring Chloramphenicol Resistance inMycoplasma pneumoniae
| Autor: | Duncan C. Krause, Tae-Wook Hahn, Elizabeth A. Mothershed, Robert H. Waldo |
|---|---|
| Rok vydání: | 1999 |
| Předmět: |
Chloramphenicol O-Acetyltransferase
Genetic Markers Staphylococcus aureus Mycoplasma pneumoniae Chloramphenicol Resistance Substrate (chemistry) Biology medicine.disease_cause Microbiology Chloramphenicol acetyltransferase Minimum inhibitory concentration DNA Transposable Elements medicine Feasibility Studies Gentamicin Cloning Molecular Gentamicins Molecular Biology Gene Selectable marker medicine.drug |
| Zdroj: | Plasmid. 41:120-124 |
| ISSN: | 0147-619X |
| DOI: | 10.1006/plas.1998.1387 |
| Popis: | The Staphylococcus aureus transposon Tn4001 and derivatives thereof have been transformed successfully in several mycoplasma species. In order to expand the versatility of Tn4001 for other genetic manipulations and for use in mycoplasma species resistant to gentamicin (Gm), chloramphenicol acetyltransferase (Cat) from S. aureus was evaluated as a selectable marker. The cat gene was cloned in both orientations into a modified Tn4001 and transformed into Mycoplasma pneumoniae, conferring resistance to Cm and Gm. Replacement of the gene for GmR in Tn4001 with cat likewise conferred CmR when transformed into M. pneumoniae. The minimum inhibitory concentration to Cm in transformants with cat derivatives of Tn4001 was 300-500 microg/ml, and Cat enzyme activity was demonstrated by using a fluorescent substrate. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect