Pipette tip dummy molecularly imprinted solid-phase extraction of Bisphenol A from urine samples and analysis by gas chromatography coupled to mass spectrometry
| Autor: | Tamires Amabile Valim Brigante, Israel Donizeti de Souza, Luis Felippe Cabral Miranda, Maria Eugênia Costa Queiroz, Vinicius Ricardo Acquaro Junior |
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| Rok vydání: | 2017 |
| Předmět: |
Polybrominated Biphenyls
Clinical Biochemistry 02 engineering and technology Mass spectrometry 01 natural sciences Biochemistry Gas Chromatography-Mass Spectrometry Analytical Chemistry Molecular Imprinting chemistry.chemical_compound Phenols Limit of Detection Humans Solid phase extraction Benzhydryl Compounds Detection limit Chromatography Propylamines Solid Phase Extraction 010401 analytical chemistry Molecularly imprinted polymer Reproducibility of Results Cell Biology General Medicine Silanes 021001 nanoscience & nanotechnology 0104 chemical sciences chemistry Linear Models Tetrabromobisphenol A COMPOSTOS FENÓLICOS Gas chromatography Gas chromatography–mass spectrometry 0210 nano-technology Molecular imprinting |
| Zdroj: | Repositório Institucional da USP (Biblioteca Digital da Produção Intelectual) Universidade de São Paulo (USP) instacron:USP |
| ISSN: | 1570-0232 |
| DOI: | 10.1016/j.jchromb.2017.09.038 |
| Popis: | Molecularly imprinted polymers (MIPs) were synthesized and used as sorbent for Bisphenol A (BPA) pipette tip solid-phase microextraction from urine samples and BPA analysis by gas chromatography coupled to mass spectrometry (GC-MS). The MIPs were synthesized by the sol-gel methodology. Aminopropyltriethoxysilane (APTES) and tetraethyl orthosilicate (TEOS) were used as functional monomer and cross-linking reagent, respectively. BPA and tetrabromobisphenol A (TBBPA) were evaluated as template during MIP synthesis. The BPA-based MIP displayed slightly higher extraction efficiency than the TBBPA-based dummy molecularly imprinted polymer (DMIP), but the TBBPA-based DMIP was selected as sorbent to minimize interference from leaked template. Comparison of the TBBPA-based DMIP, BPA-based MIP, and non-imprinted polymer (NIP) extraction efficiencies attested that the TBBPA-based DMIP was selective. The synthesized polymers were characterized by Scanning Electron Microscopy (SEM) and Fourier Transform Infrared spectroscopy (FTIR). The TBBPA-based DMIP was reused for over 100 times, which confirmed its robustness. The developed method was linear from 50 to 500ngmL-1. Precision values had coefficient of variation (CV) ranging from 4 to 14%. The accuracy relative standard deviation values (RSD) varied from -13.6 to 12.3%. |
| Databáze: | OpenAIRE |
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