The distribution of motor proteins in the muscles and filame cells of the Schistosoma mansoni miracidium and primary sporocyst
| Autor: | D. Cioli, Guilherme Oliveira, Diana Bahia, F. Vigorosi, Renato A. Mortara, Lívia Avelar |
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| Rok vydání: | 2006 |
| Předmět: |
Antibodies
Helminth Motility Myosins Filamentous actin Motor protein Myosin Fluorescence microscope Animals Parasite hosting Actin Life Cycle Stages Microscopy Confocal biology Molecular Motor Proteins Muscles Oocysts Schistosoma mansoni Anatomy biology.organism_classification Actins Cell biology Actin Cytoskeleton Infectious Diseases Animal Science and Zoology Parasitology |
| Zdroj: | Parasitology (Lond., Print) 133 (2006): 321–329. info:cnr-pdr/source/autori:Bahia D, Avelar LGA, Vigorosi F, Cioli D, Oliveira GC, Mortara RA/titolo:The distribution of motor proteins in the muscles and filame cells of the Schistosoma mansoni miracidium and primary sporocyst./doi:/rivista:Parasitology (Lond., Print)/anno:2006/pagina_da:321/pagina_a:329/intervallo_pagine:321–329/volume:133 |
| Popis: | Schistosoma mansoni eggs, miracidia and primary sporocysts were labelled with phalloidin-rhodamine to visualize filamentous actin structures. Analysis of these forms by confocal fluorescence microscopy revealed the presence of previously well-defined circular and longitudinal muscle layers. Besides these muscular layers that sustain and provide motility to these parasite forms, we found in these 3 consecutive developmental stages of the parasite previously unidentified actin-rich tubular structures. In the 3 forms, 4 actin-rich tubules could be observed by optical sectioning underneath the well-developed muscle layers. The tubules appear in pairs, transversal to the length of the parasite, and located towards the extremities. By using an anti-flame cell specific antibody we confirmed that the tubules co-localize with flame cells and also determined that the tubule core is filled with microtubules. The additional presence of myosin in these tubules strongly suggests that they are contractile structures. |
| Databáze: | OpenAIRE |
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