Venturi easy ambient sonic-spray ionization
| Autor: | Fernando Coelho, Marcos N. Eberlin, Jose Luis Paz Jara, Thaís Regiani, Vanessa G. Santos, Wanderson Romão, Fernanda Furlan Gonçalves Dias, Clécio F. Klitzke |
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| Rok vydání: | 2011 |
| Předmět: |
Ions
Chemistry Myoglobin Analytical chemistry Thermal ionization Cytochromes c Mass spectrometry Mass Spectrometry Analytical Chemistry Solvent Sound Fragmentation (mass spectrometry) Ionization Desorption Venturi effect Animals Amino Acid Sequence Gases Organic Chemicals Oligopeptides Ambient ionization |
| Zdroj: | Analytical chemistry. 83(4) |
| ISSN: | 1520-6882 |
| Popis: | The development and illustrative applications of an ambient ionization technique termed Venturi easy ambient sonic-spray ionization (V-EASI) is described. Its dual mode of operation with Venturi self-pumping makes V-EASI applicable to the direct mass spectrometric analysis of both liquid (V(L)-EASI) and solid (V(S)-EASI) samples. V-EASI is simple and easy to assemble, operating solely via the assistance of a sonic stream of nitrogen or air. The sonic gas stream causes two beneficial and integrated effects: (a) the self-pumping of solutions via the Venturi effect and (b) sonic-spray ionization (SSI) of analytes either in solution or resting on solid surfaces. In its liquid mode, V(L)-EASI is applicable to analytes in solution, forming negatively and/or positively charged intact molecular species in a soft fashion with little or no fragmentation. In its solid mode, V(S)-EASI relies on Venturi self-pumping of a proper SSI solvent solution in combination with SSI to form a stream of bipolar charged droplets that bombard the sample surface, causing desorption and ionization of the analyte molecules. As for its precursor technique (EASI), V-EASI generates bipolar droplets with considerably lower average charging, which increases selectivity for ionization with high signal-to-noise ratios and clean spectra dominated by single molecular species with minimal solvent ions. V-EASI also operates in a voltage-, heat-, and radiation-free fashion and is therefore free of thermal, electrical, or discharge interferences. |
| Databáze: | OpenAIRE |
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