Development of a population suppression strain of the human malaria vector mosquito, Anopheles stephensi
| Autor: | Karissa Chow, Guoliang Fu, Nijole Jasinskiene, David M. Brown, Osvaldo Marinotti, Aniko Fazekas, Anthony A. James, Sarah Scaife, Luke Alphey, Stefanie T Mattingly |
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| Jazyk: | angličtina |
| Rok vydání: | 2013 |
| Předmět: |
Male
Mosquito Control Population Expression Aedes aegypti Anopheles Stephensi Transgenic Mosquitoes Gene Animals Genetically Modified Antibiotics parasitic diseases Anopheles medicine Medicine and Health Sciences Animals Humans Malaria Phosphatase Type-1 Pp1 Pest Control Biological education Anopheles stephensi Recombination Genetic Genetics education.field_of_study biology Research Life Sciences Aedes-Aegypti biology.organism_classification medicine.disease Phenotype Malaria Mosquito control Infectious Diseases Transgenic mosquitoes Vector (epidemiology) Gene Targeting DNA Transposable Elements Female Parasitology Drosophila Locomotion |
| Zdroj: | Marinotti, Osvaldo; Jasinskiene, Nijole; Fazekas, Aniko; Scaife, Sarah; Fu, Guoliang; Mattingly, Stefanie T; et al.(2013). Development of a population suppression strain of the human malaria vector mosquito, Anopheles stephensi. Malaria Journal, 12(1), 142. doi: 10.1186/1475-2875-12-142. UC Irvine: Retrieved from: http://www.escholarship.org/uc/item/2nq8p7kb Malaria Journal Marinotti, Osvaldo; Jasinskiene, Nijole; Fazekas, Aniko; Scaife, Sarah; Fu, Guoliang; Mattingly, Stefanie T; et al.(2013). Development of a population suppression strain of the human malaria vector mosquito, Anopheles stephensi. Malaria Journal, 12(1), 142. doi: http://dx.doi.org/10.1186/1475-2875-12-142. Retrieved from: http://www.escholarship.org/uc/item/8bp46066 |
| DOI: | 10.1186/1475-2875-12-142. |
| Popis: | Background Transgenic mosquito strains are being developed to contribute to the control of dengue and malaria transmission. One approach uses genetic manipulation to confer conditional, female-specific dominant lethality phenotypes. Engineering of a female-specific flightless phenotype provides a sexing mechanism essential for male-only mosquito, release approaches that result in population suppression of target vector species. Methods An approach that uses a female-specific gene promoter and antibiotic-repressible lethal factor to produce a sex-specific flightless phenotype was adapted to the human malaria vector, Anopheles stephensi. Transposon- and site-specific recombination-mediated technologies were used to generate a number of transgenic An. stephensi lines that when combined through mating produced the phenotype of flight-inhibited females and flight-capable males. Results The data shown here demonstrate the successful engineering of a female-specific flightless phenotype in a malaria vector. The flightless phenotype was repressible by the addition of tetracycline to the larval diet. This conditional phenotype allows the rearing of the strains under routine laboratory conditions. The minimal level of tetracycline that rescues the flightless phenotype is higher than that found as an environmental contaminant in circumstances where there is intensive use of antibiotics. Conclusions These studies support the further development of flightless female technology for applications in malaria control programmes that target the vectors. |
| Databáze: | OpenAIRE |
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