Targets for SMR1-pentapeptide suggest a link between the circulating peptide and mineral transport
| Autor: | L. Le Doledec, A. Cardona, J. M. Grognet, Catherine Rougeot, R. Vienet, F. Rougeon |
|---|---|
| Rok vydání: | 1997 |
| Předmět: |
Male
medicine.medical_specialty Aging Time Factors Physiology Peptide Nephron Biology Bone tissue Pentapeptide repeat In vivo Physiology (medical) Internal medicine medicine Animals Tissue Distribution Protein Precursors Rats Wistar Salivary Proteins and Peptides chemistry.chemical_classification Kidney Minerals Sex Characteristics Binding Sites Biological Transport Submandibular gland Cell biology Rats medicine.anatomical_structure Endocrinology chemistry Autoradiography Female Homeostasis |
| Zdroj: | The American journal of physiology. 273(4) |
| ISSN: | 0002-9513 |
| Popis: | The submandibular rat 1 protein (SMR1) is selectively processed at pairs of basic amino acid residues in a tissue- and sex-specific manner. We have mapped peripheral targets for the final secretory maturation product of SMR1, the pentapeptide QHNPR, by examining in vivo the tissue distribution of the radiolabeled peptide using β-radio imager whole body autoradiography. The characteristics of tissue uptake allowed specific binding sites at physiological peptide concentrations to be identified within the renal outer medulla, bone and dental tissue, glandular gastric mucosa, and pancreatic lobules. Direct evidence that pentapeptide binding sites are localized in selective portions of the male rat nephron, within the S3, S2, and S1 segments of the proximal tubules, was obtained. In bone tissue the pentapeptide exclusively accumulates within the trabecular bone remodeling unit, and in dental tissue it concentrates within the tubules of the dentinal rat incisor. In relation to male rat-specific behavioral characteristics, our data suggest that the circulating androgen-regulated SMR1-derived pentapeptide is primarily involved in the modulation of mineral balance between at least four systems: kidney, bone, tooth, and circulation. |
| Databáze: | OpenAIRE |
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