Fast Photo-Chrono-Amperometry of Photosynthetic Complexes for Biosensors and Electron Transport Studies
| Autor: | Maria Elena Antinori, Pau Gorostiza, Roberta Croce, Niek F. van Hulst, Chen Hu, Manuel López-Ortiz, Vikas Remesh, Ricardo A. Zamora |
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| Přispěvatelé: | Biophysics Photosynthesis/Energy, LaserLaB - Energy |
| Rok vydání: | 2021 |
| Předmět: |
Paraquat
Photosystem II Plastoquinone Bioengineering 02 engineering and technology Biosensing Techniques Photochemistry Photosystem I Photosynthesis 01 natural sciences Redox Electron Transport chemistry.chemical_compound medicine Instrumentation Fluid Flow and Transfer Processes Física [Àrees temàtiques de la UPC] Photosystem I Protein Complex Process Chemistry and Technology 010401 analytical chemistry Photosystem II Protein Complex Viologen 021001 nanoscience & nanotechnology Electron transport chain 0104 chemical sciences Biosensors chemistry 0210 nano-technology Biosensor medicine.drug |
| Zdroj: | UPCommons. Portal del coneixement obert de la UPC Universitat Politècnica de Catalunya (UPC) López-Ortiz, M, Zamora, R A, Antinori, M E, Remesh, V, Hu, C, Croce, R, Van Hulst, N F & Gorostiza, P 2021, ' Fast Photo-Chrono-Amperometry of Photosynthetic Complexes for Biosensors and Electron Transport Studies ', ACS Sensors, vol. 6, no. 2, pp. 581-587 . https://doi.org/10.1021/acssensors.1c00179 ACS Sensors, 6(2), 581-587. American Chemical Society |
| ISSN: | 2379-3694 |
| Popis: | Photosynthetic reactions in plants, algae, and cyanobacteria are driven by photosystem I and photosystem II complexes, which specifically reduce or oxidize partner redox biomolecules. Photosynthetic complexes can also bind synthetic organic molecules, which inhibit their photoactivity and can be used both to study the electron transport chain and as herbicides and algicides. Thus, their development, characterization, and sensing bears fundamental and applied interest. Substantial efforts have been devoted to developing photosensors based on photosystem II to detect compounds that bind to the plastoquinone sites of this complex. In comparison, photosystem I based sensors have received less attention and could be used to identify novel substances displaying phytotoxic effects, including those obtained from natural product extracts. We have developed a robust procedure to functionalize gold electrodes with photo- and redox-active photosystem I complexes based on transparent gold and a thiolate self-assembled monolayer, and we have obtained reproducible electrochemical photoresponses. Chronoamperometric recordings have allowed us to measure photocurrents in the presence of the viologen derivative paraquat at concentrations below 100 nM under lock-in operation and a sensor dynamic range spanning six orders of magnitude up to 100 mM. We have modeled their time course to identify the main electrochemical processes and limiting steps in the electron transport chain. Our results allow us to isolate the contributions from photosystem I and the redox mediator, and evaluate photocurrent features (spectral and power dependence, fast transient kinetics) that could be used as a sensing signal to detect other inhibitors and modulators of photosystem I activity. |
| Databáze: | OpenAIRE |
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