Aphidicolin induces myogenic differentiation in the human rhabdomyosarcoma cell line KFR
| Autor: | Bernhard Kornhuber, Milos Novak, Jindrich Cinatl, Jaroslav Cinatl, Holger F. Rabenau, Pablo Hernáiz Driever, Milan Stefanik, Hans-Wilhelm Doerr |
|---|---|
| Rok vydání: | 1994 |
| Předmět: |
Aphidicolin
DNA Replication Cell division Cell Survival Cellular differentiation Biology Myosins chemistry.chemical_compound medicine Tumor Cells Cultured Humans Rhabdomyosarcoma Alveolar Cell fusion DNA synthesis Cell growth Muscles Cell Differentiation Cell Biology General Medicine medicine.disease Molecular biology chemistry Cell culture Immunology Alveolar rhabdomyosarcoma Cell Division |
| Zdroj: | Cell biology international. 18(4) |
| ISSN: | 1065-6995 |
| Popis: | The effects of aphidicolin, a specific inhibitor of DNA polymerase alpha, on cell growth, DNA synthesis and myogenic differentiation in the human alveolar rhabdomyosarcoma cell line KFR were studied. The treatment with aphidicolin at 5 x 10(-6) M concentration, which completely inhibited DNA synthesis and cell growth, induced morphological differentiation of small mononuclear cells to elongated, multinucleated (myotube-like) structures. The morphological differentiation was accompanied by the expression of skeletal muscle myosin; about 30% myosin-positive cells were observed after 14 days of treatment, compared to 2.3% in untreated cultures. The results showed that aphidicolin induces differentiation of human rhabdomyosarcoma cells and that multinucleated myotube-like elements may develop simply by cell fusion without cell division and DNA synthesis. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Plný text