Possible effects of melatonin against rat uterus exposure to bisphenol A during neonatal period
| Autor: | Bahar Kartal, Vildan Aydin, Neslihan Coşkun Akçay, Damla Dernek, Suna Ömeroğlu, Saadet Özen Akarca Dizakar, İsmail Türkoğlu |
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| Jazyk: | angličtina |
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
medicine.medical_specialty endocrine system Health Toxicology and Mutagenesis Uterus Apoptosis Biology Melatonin 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Phenols Internal medicine medicine Animals Environmental Chemistry Benzhydryl Compounds 030219 obstetrics & reproductive medicine Ethanol TUNEL assay urogenital system Cytochrome c Cytochromes c Histology General Medicine Pollution Rats 030104 developmental biology medicine.anatomical_structure Endocrinology Animals Newborn Proto-Oncogene Proteins c-bcl-2 chemistry biology.protein Environmental Pollutants Female Immunostaining hormones hormone substitutes and hormone antagonists medicine.drug |
| Popis: | The aim of this study was to investigate the possible effects of melatonin on rat uterine tissue against exposure with bisphenol A (BPA) in the neonatal period. Twenty-four female rats were divided into four groups, (n= 6) per group. Group I was used as a control (sesame oil + ethanol), group II was injected daily with (100 mg/kg) BPA by subcutaneously (sc) daily postnatal days (PND 0-10), group III was injected daily with (10 mg/kg) melatonin by sc for 10 days (PND 20-30), and group IV was injected daily with (100 mg/kg) BPA (PND 0-10) and (10 mg/kg) melatonin (PND 20-30). All rats were sacrificed in the same day of metestrus cycle, approximately PND 70. Histological analyses, immunostaining of B cell lymphoma 2 (Bcl-2), and cytochrome c and TUNEL assays were performed. According to our results, neonatal exposure to BPA accelerates onset of puberty, causes degenerative and morphometric changes on rat uterus, and increases apoptotic reaction rates. The immunoreactivity of Bcl-2 was decreased after BPA administration. In addition, immunoreactivity of Bcl-2 showed an increase after melatonin treatment. However, cytochrome c immunoreactivity was decreased after melatonin administration. Our results suggest that melatonin may have positive effects against BPA-induced degenerative changes on rat uterus. |
| Databáze: | OpenAIRE |
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