Enhanced Internalization of Indian Ayurvedic Swarna Bhasma (Gold Nanopowder) for Effective Interaction with Human Cells
| Autor: | Kiran Kuruvinashetti, Ahmad Sohrabi Kashani, Muthukumaran Packirisamy, Simona Badilescu, Daniel Beauet, Alisa Piekny |
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| Rok vydání: | 2018 |
| Předmět: |
Materials science
Latex Cell Survival 020209 energy media_common.quotation_subject Biomedical Engineering Metal Nanoparticles Nanoparticle Bioengineering 02 engineering and technology Cellular viability Arsenic Cell Line HeLa 0202 electrical engineering electronic engineering information engineering Humans General Materials Science Trituration Particle Size Internalization Roasting media_common biology Spectral properties General Chemistry 021001 nanoscience & nanotechnology Condensed Matter Physics biology.organism_classification Medicine Ayurvedic Drug Combinations Calotropis Lead Colloidal gold Gold 0210 nano-technology HeLa Cells Nuclear chemistry |
| Zdroj: | Journal of Nanoscience and Nanotechnology. 18:6791-6798 |
| ISSN: | 1533-4880 |
| DOI: | 10.1166/jnn.2018.15503 |
| Popis: | In the ancient traditional Indian Ayurvedic system of natural healing, gold nanoparticles (Swarna Bhasma, gold ash) have been used for its therapeutic benefits as far back as 2500 B.C. Ayurvedic medicinal preparations are complex mixtures that include many plant-derived products and metals. Bhasmas date as far back as the 8th century and are made by samskaras (processings), such as shodhana (purification and potentiation), jarana (roasting), and marana (incineration, trituration) in the presence of plant products, including juices and concoctions. Previous studies characterized the physical properties of gold ash, and the mechanisms of its entry into human cells, but only preliminary data exist on its toxicity. Before using nanoparticles for therapeutic application, it is extremely important to study their toxicity and cellular internalization. In the present study, various imaging techniques were used to investigate Swarna Bhasma's (gold nanopowder) toxicity in both cancerous and noncancerous cells (HeLa and HFF-1) and to characterize its spectral properties. The results showed that gold ash particles had no impact on the cellular viability of both HeLa and HFF-1 cells, even at high concentrations or long incubation times. Moreover, it was found that the internalization level of Swarna Bhasma to cells may be improved by mechanical breaking of the large aggregates into smaller agglomerates. Hyperspectral images revealed that after breaking, the small agglomerates have different spectral properties in cells, compared to the original aggregates, suggesting that size of particles is instrumental for the subcellular interaction with human cells. |
| Databáze: | OpenAIRE |
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