Replication of the Zika virus in different iPSC-derived neuronal cells and implications to assess efficacy of antivirals
Autor: | Johan Neyts, Kristel Eggermont, Suzanne J.F. Kaptein, Catherine M. Verfaillie, Leen Delang, Kristina Lanko, Abdulsamie Patel |
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Jazyk: | angličtina |
Rok vydání: | 2017 |
Předmět: |
0301 basic medicine
DNA Replication viruses 030106 microbiology Induced Pluripotent Stem Cells Favipiravir Virus Replication Antiviral Agents Virus Zika virus Cell Line 03 medical and health sciences Virology Chlorocebus aethiops Ribavirin Animals Humans Induced pluripotent stem cell Vero Cells Pharmacology Neurons biology Zika Virus biology.organism_classification 3. Good health 030104 developmental biology Viral replication Cell culture Vero cell Stem cell |
Zdroj: | Antiviral Research Lirias |
Popis: | Infections with the Zika virus (ZIKV) are responsible for congenital abnormalities and neurological disorders. We here demonstrate that ZIKV productively infects three types of human iPSC (induced pluripotent stem cells)-derived cells from the neural lineage, i.e. cortical and motor neurons as well as astrocytes. ZIKV infection results in all three cell types in the production of infectious virus particles and induces cytopathic effects (CPE). In cortical and motor neurons, an Asian isolate (PRVABC59) produced roughly 10-fold more virus than the prototypic African strain (MR766 strain). Viral replication and CPE is efficiently inhibited by the nucleoside polymerase inhibitor 7-deaza-2'-C-methyladenosine (7DMA). However, ribavirin and favipiravir, two molecules that inhibit ZIKV replication in Vero cells, did not inhibit ZIKV replication in the neuronal cells. These results highlight the need to assess the potential antiviral activity of novel ZIKV inhibitors in stem cell derived neuronal cultures. ispartof: ANTIVIRAL RESEARCH vol:145 pages:82-86 ispartof: location:Netherlands status: published |
Databáze: | OpenAIRE |
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