bbeaR: an R package and framework for epitope-specific antibody profiling
| Autor: | Hugh A. Sampson, Mayte Suárez-Fariñas, Randall J. Ellis, Maria Suprun |
|---|---|
| Rok vydání: | 2021 |
| Předmět: |
Statistics and Probability
Computer science Computational biology Immunoglobulin E Biochemistry Immunoglobulin D Epitope 03 medical and health sciences 0302 clinical medicine Protein structure medicine Multiplex Molecular Biology 030304 developmental biology chemistry.chemical_classification Profiling (computer programming) 0303 health sciences biology medicine.diagnostic_test Applications Notes Computer Science Applications Amino acid Computational Mathematics Specific antibody R package 030228 respiratory system Computational Theory and Mathematics chemistry Immunoassay biology.protein Antibody |
| Zdroj: | Bioinformatics |
| ISSN: | 1367-4811 1367-4803 |
| DOI: | 10.1093/bioinformatics/btaa1064 |
| Popis: | Summary Analysis of epitope-specific antibody repertoires has provided novel insights into the pathogenesis of inflammatory disorders, especially allergies. A novel multiplex immunoassay, termed Bead-Based Epitope Assay (BBEA), was developed to quantify levels of epitope-specific immunoglobulins, including IgE, IgG, IgA and IgD isotypes. bbeaR is an open-source R package, developed for the BBEA, provides a framework to import, process and normalize .csv data files exported from the Luminex reader, evaluate various quality control metrics, analyze differential epitope-binding antibodies with linear modeling, visualize results and map epitopes’ amino acid sequences to their respective primary protein structures. bbeaR enables streamlined and reproducible analysis of epitope-specific antibody profiles. Availability and implementation bbeaR is open-source and freely available from GitHub as an R package: https://github.com/msuprun/bbeaR; vignettes included. Supplementary information Supplementary data are available at Bioinformatics online. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|