Differential gene expression between in vivo and in vitro maturation: a comparative study with bovine oocytes derived from the same donor pool
| Autor: | Michele Munk, João Henrique Moreira Viana, Luiz Sérgio de Almeida Camargo, José Nelio de Sousa Sales, S. Wohlres-Viana, C. C. R. Quintão |
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| Přispěvatelé: | LUIZ SERGIO DE ALMEIDA CAMARGO, CNPGL, MICHELE MUNK, UFJF, JOSE NELIO SALES, UFLA, SABINE WOHLRES-VIANA, UFJF, CAROLINA CAPOBIANGO ROMANO QUINTAO, CNPGL, JOAO HENRIQUE MOREIRA VIANA, Cenargen. |
| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
mRNA ovum pick-up Biology Peroxiredoxin 1 In vivo maturation Andrology 03 medical and health sciences 0302 clinical medicine In vivo Complementary DNA Gene expression MRNA medicine Animals RNA Messenger Messenger RNA 030219 obstetrics & reproductive medicine Embryo in vivo maturation Oocyte In Vitro Oocyte Maturation Techniques In vitro maturation 030104 developmental biology medicine.anatomical_structure gene expression Oocytes Original Article Cattle Female Transcriptome Ovum pick-up |
| Zdroj: | Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA-Alice) Empresa Brasileira de Pesquisa Agropecuária (Embrapa) instacron:EMBRAPA JBRA Assisted Reproduction |
| ISSN: | 1518-0557 |
| DOI: | 10.5935/1518-0557.20180084 |
| Popis: | Objective: In vitro maturation has been shown to influence gene expression in oocytes, but a common shortcoming in reports on the matter has been the use of different donors in each experimental group thus disregarding donor effects. This study aimed to investigate the abundance of mRNA in oocytes matured in vivo and in vitro obtained from the same group of donors. Methods: A bovine model was used to assess the relative abundance of specific transcripts in in vitro-matured (IN VITRO-OPU) and in vivo-matured (IN VIVO-OPU) oocytes collected from the same donors by transvaginal ovum pick-up (OPU). Transcript abundance in oocytes from the IN VIVO-OPU group and oocytes matured in vitro but retrieved from different cows slaughtered at a commercial abattoir (IN VITRO-Abattoir group) was also compared. Total RNA was extracted from denuded oocytes and cDNA was produced via reverse transcription using an oligo(dT) primer for relative quantification of eight target transcripts by real-time PCR. Results: Oocytes in the IN VITRO-OPU group had lower (p |
| Databáze: | OpenAIRE |
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