The C-Terminal Heptapeptide of Bombesin Reduces the Deleterious Effect of Total Parenteral Nutrition (TPN) on Gut-Associated Lymphoid Tissue (GALT) Mass but Not Intestinal Immunoglobulin AIn Vivo
| Autor: | Shannon R. Reese, Kenneth A. Kudsk, Shigeo Ikeda, Laurence Genton, Tho Le |
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| Rok vydání: | 2004 |
| Předmět: |
Male
0301 basic medicine Immunoglobulin A medicine.medical_specialty Lymphoid Tissue Lymphocyte Gut-associated lymphoid tissue Medicine (miscellaneous) Enzyme-Linked Immunosorbent Assay Enteral administration Mice Peyer's Patches Random Allocation Structure-Activity Relationship 03 medical and health sciences chemistry.chemical_compound Internal medicine medicine Animals Lymphocyte Count Intestinal Mucosa Analysis of Variance Mice Inbred ICR Lamina propria 030109 nutrition & dietetics Nutrition and Dietetics Dose-Response Relationship Drug biology Bombesin 030104 developmental biology medicine.anatomical_structure Parenteral nutrition Endocrinology Lymphatic system chemistry biology.protein Parenteral Nutrition Total |
| Zdroj: | Journal of Parenteral and Enteral Nutrition. 28:431-434 |
| ISSN: | 1941-2444 0148-6071 |
| Popis: | Bombesin, the amphibian analog of mammalian gastrin-releasing peptide, reverses total parenteral nutrition (TPN)-induced atrophy of gut-associated lymphoid tissue and increases intestinal and respiratory immunoglobulin A (IgA) levels. Structure-activity studies suggested that the biologically active portion of bombesin is a C-terminal heptapeptide (7AA). This study investigates the effect of 7AA on lymphocytes counts of the Peyer's patches (PP), the lamina propria (LP) and the intraepithelial layer (IE).Forty-eight male mice were randomized to receive chow (n = 13), TPN only (n = 9), TPN + 15 microg 7AA 3 times per day (n = 13) or TPN + 150 microg 7AA 3 times per day (n = 13). After 5 days of feeding, PP, LP, and IE lymphocytes were determined. Intestinal IgA levels were measured with ELISA. Groups were compared with ANOVA.All TPN-fed mice lost more weight than mice fed chow (p.04). Lymphocyte counts in PP, LP, and IE were significantly lower in the TPN group than in the 3 other groups but did not differ between the groups fed chow, TPN + 15 microg 7AA 3 times per day, or TPN + 150 microg 7AA 3 times per day. Intestinal IgA levels were higher in chow-fed mice (148.4 +/- 16.9) than in mice fed TPN (98.4 +/- 14.0, p = .008), TPN + 15 microg 7AA 3 times per day (96.9 +/- 7.7, p = .003) or TPN + 150 microg 7AA 3 times per day (87.3 +/- 6.7, p = .001).The C-terminal heptapeptide of bombesin prevented the TPN-induced decrease in intestinal lymphocyte populations but not the reduction in intestinal IgA levels. |
| Databáze: | OpenAIRE |
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