Identification and characterization of influenza A viruses in selected domestic animals in Kenya, 2010-2012

Autor: Joshua A. Mott, Lilian W. Waiboci, Philip Kitala, M. Kariuki Njenga, Geoffrey Arunga, P N Nyaga, Lydia Mwasi, Carol J. Cardona, Peninah Munyua, Barry S. Fields, Clayton Onyango
Rok vydání: 2018
Předmět:
RNA viruses
0301 basic medicine
Viral Diseases
Veterinary medicine
Swine
lcsh:Medicine
Hemagglutinin Glycoproteins
Influenza Virus
medicine.disease_cause
Poultry
Influenza A Virus
H1N1 Subtype
Influenza A virus
Gamefowl
Enzyme-Linked Immunoassays
lcsh:Science
Phylogeny
Pathology and laboratory medicine
Mammals
Multidisciplinary
Eukaryota
virus diseases
Medical microbiology
Subtyping
Titer
Infectious Diseases
Animals
Domestic
Vertebrates
Viruses
Nasal Cavity
Pathogens
Antibody
Research Article
030106 microbiology
Hemagglutinin (influenza)
Enzyme-Linked Immunosorbent Assay
Biology
Research and Analysis Methods
Microbiology
Virus
Birds
03 medical and health sciences
Dogs
Orthomyxoviridae Infections
medicine
Animals
Influenza viruses
Seroprevalence
Immunoassays
Medicine and health sciences
Hemagglutination assay
lcsh:R
Organisms
Viral pathogens
Biology and Life Sciences
Kenya
Influenza
Microbial pathogens
030104 developmental biology
Fowl
Amniotes
Immunologic Techniques
Cats
biology.protein
lcsh:Q
Chickens
Orthomyxoviruses
Zdroj: PLoS ONE, Vol 13, Iss 2, p e0192721 (2018)
PLoS ONE
ISSN: 1932-6203
DOI: 10.1371/journal.pone.0192721
Popis: Background Influenza A virus subtypes in non-human hosts have not been characterized in Kenya. We carried out influenza surveillance in selected domestic animals and compared the virus isolates with isolates obtained in humans during the same period. Methods We collected nasal swabs from pigs, dogs and cats; oropharyngeal and cloacal swabs from poultry; and blood samples from all animals between 2010 and 2012. A standardized questionnaire was administered to farmers and traders. Swabs were tested for influenza A by rtRT-PCR, virus isolation and subtyping was done on all positive swabs. All sera were screened for influenza A antibodies by ELISA, and positives were evaluated by hemagglutination inhibition (HI). Full genome sequencing was done on four selected pig virus isolates. Results Among 3,798 sera tested by ELISA, influenza A seroprevalence was highest in pigs (15.9%; 172/1084), 1.2% (3/258) in ducks, 1.4% (1/72) in cats 0.6% (3/467) in dogs, 0.1% (2/1894) in chicken and 0% in geese and turkeys. HI testing of ELISA-positive pig sera showed that 71.5% had positive titers to A/California/04/2009(H1N1). Among 6,289 swabs tested by rRT-PCR, influenza A prevalence was highest in ducks [1.2%; 5/423] and 0% in cats and turkeys. Eight virus isolates were obtained from pig nasal swabs collected in 2011 and were determined to be A(H1N1)pdm09 on subtyping. On phylogenetic analysis, four hemagglutinin segments from pig isolates clustered together and were closely associated with human influenza viruses that circulated in Kenya in 2011. Conclusion Influenza A(H1N1)pdm09 isolated in pigs was genetically similar to contemporary human pandemic influenza virus isolates. This suggest that the virus was likely transmitted from humans to pigs, became established and circulated in Kenyan pig populations during the study period. Minimal influenza A prevalence was observed in the other animals studied.
Databáze: OpenAIRE
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