Cloning, bioinformatics and the enzyme activity analyses of a phenylalanine ammonia-lyase gene involved in dragon’s blood biosynthesis in Dracaena cambodiana
| Autor: | Changhe Zhang, Ji-Dong Lou, Shui Zheng, Lingcheng Ou, José Gomes-Laranjo, Liang Tang, Xing-Hong Wang, Min Gong |
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| Rok vydání: | 2012 |
| Předmět: |
Sequence analysis
Molecular Sequence Data Phenylalanine ammonia-lyase Biology Bioinformatics Genetics Amino Acid Sequence Cloning Molecular Codon Molecular Biology Gene Dracaena Phylogeny Phenylalanine Ammonia-Lyase Base Sequence Phenylpropanoid Computational Biology Sequence Analysis DNA General Medicine biology.organism_classification humanities Protein Structure Tertiary Enzyme Activation Open reading frame Codon usage bias Hydrophobic and Hydrophilic Interactions GC-content |
| Zdroj: | Molecular Biology Reports. 40:97-107 |
| ISSN: | 1573-4978 0301-4851 |
| DOI: | 10.1007/s11033-012-2032-y |
| Popis: | Phenylalanine ammonia-lyase (PAL) is the key enzyme of the phenylpropanoid pathway, playing an important role in plant development and defence. We cloned a partial cDNA of PAL gene, DcPAL1, from Dracaena cambodiana seedlings using RT-PCR with degenerate primers that were designed based on a multiple sequence alignment of known PAL genes from other plant species. DcPAL1 shows highly homologous to other known PAL genes registered in GenBank, being closest to that of Musa acuminata. DcPAL1 has a relatively high GC content and most of the GC is in the third codon position. It has 768 bp in size with a maximum open reading frame (ORF) of 765 bp, encoding a 255 amino acid-polypeptide. The deduced PAL protein is a stable protein, having classical PAL domains and consisting of three major hydrophobic domains. Analysis of effective number of codons (ENC) shows that DcPAL1 codons are used at equal frequency. Relatively higher usage frequency appears randomly in codons ended with any of the four bases; six codons have no usage bias. There are 45 codons showing distinct usage preference between DcPAL1 and E. coli, 20 between DcPAL1 and yeast. Therefore, the yeast system may be more suitable for the expression of DcPAL1. Upon the elicitation of Fusarium proliferatum, a potent elicitor of dragon’s blood, the PAL enzyme activity in the leaves and stems of D. cambodiana and other two Dracaena spp. significantly increased, accompanying with the formation of dragon’s blood, indicating the involvement of PAL in the biosynthesis of dragon’s blood, a precious traditional medicine. |
| Databáze: | OpenAIRE |
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