Microwave-Synthesized Platinum-Embedded Mesoporous Silica Nanoparticles as Dual-Modality Contrast Agents: Computed Tomography and Optical Imaging
| Autor: | Wei-Neng Liao, Shih-Hsun Cheng, Leu-Wei Lo, Nai-Tzu Chen, Chia-Hui Chu |
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| Rok vydání: | 2018 |
| Předmět: |
Nanoparticle
Contrast Media 02 engineering and technology Chemistry Techniques Synthetic naked platinum nanoparticles (nPtNPs) Platinum nanoparticles 01 natural sciences lcsh:Chemistry Mice Microwaves lcsh:QH301-705.5 Inductively coupled plasma mass spectrometry Spectroscopy Optical Imaging General Medicine 021001 nanoscience & nanotechnology Silicon Dioxide Fluorescence Computer Science Applications Female 0210 nano-technology Porosity Preclinical imaging Materials science chemistry.chemical_element Breast Neoplasms Conjugated system computed tomography (CT) 010402 general chemistry Catalysis Article microwave synthesis Inorganic Chemistry Cell Line Tumor mesoporous silica nanoparticles (MSNs) Animals Humans Physical and Theoretical Chemistry Molecular Biology Fluorescent Dyes Platinum dual-modality contrast agents Organic Chemistry Mesoporous silica 0104 chemical sciences lcsh:Biology (General) lcsh:QD1-999 chemistry Nanoparticles Tomography X-Ray Computed Biomedical engineering |
| Zdroj: | International Journal of Molecular Sciences International Journal of Molecular Sciences, Vol 20, Iss 7, p 1560 (2019) Volume 20 Issue 7 |
| ISSN: | 1422-0067 |
| Popis: | Nanoparticle-based imaging contrast agents have drawn tremendous attention especially in multi-modality imaging. In this study, we developed mesoporous silica nanoparticles (MSNs) for use as dual-modality contrast agents for computed tomography (CT) and near-infrared (NIR) optical imaging (OI). A microwave synthesis for preparing naked platinum nanoparticles (nPtNPs) on MSNs (MSNs-Pt) was developed and characterized with physicochemical analysis and imaging systems. The high density of nPtNPs on the surface of the MSNs could greatly enhance the CT contrast. Inductively coupled plasma mass spectrometry (ICP-MS) revealed the MSNs-Pt compositions to be ~14% Pt by weight and TEM revealed an average particle diameter of ~50 nm and covered with ~3 nm diameter nPtNPs. To enhance the OI contrast, the NIR fluorescent dye Dy800 was conjugated to the MSNs-Pt nanochannels. The fluorescence spectra of MSNs-Pt-Dy800 were very similar to unconjugated Dy800. The CT imaging demonstrated that even modest degrees of Pt labeling could result in substantial X-ray attenuation. In vivo imaging of breast tumor-bearing mice treated with PEGylated MSNs-Pt-Dy800 (PEG-MSNs-Pt-Dy800) showed significantly improved contrasts in both fluorescence and CT imaging and the signal intensity within the tumor retained for 24 h post-injection. |
| Databáze: | OpenAIRE |
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