Molecular characterization of clinical isolates of Cyclospora cayetanensis from patients with diarrhea in India
| Autor: | B R Mirdha, G K Makharia, Shinjini Bhatnagar, Prasad Yadav, Shehla Khalil |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2015 |
| Předmět: |
Microbiology (medical)
Adult Male Diarrhea Genotype Molecular Sequence Data prevalence lcsh:QR1-502 India Cyclospora cayetanensis DNA Ribosomal Polymerase Chain Reaction 18S ribosomal RNA lcsh:Microbiology law.invention Microbiology Feces law medicine RNA Ribosomal 18S Animals Cluster Analysis Humans Natural reservoir Cyclospora infection Cyclosporiasis Child Polymerase chain reaction Phylogeny Microscopy biology phylogenetic analysis Sequence Analysis DNA Ribosomal RNA DNA Protozoan biology.organism_classification medicine.disease Virology Cyclospora Child Preschool Female medicine.symptom Polymorphism Restriction Fragment Length |
| Zdroj: | Indian Journal of Medical Microbiology, Vol 33, Iss 3, Pp 351-356 (2015) |
| ISSN: | 1998-3646 0255-0857 |
| Popis: | Purpose: Cyclospora cayetanensis is an intestinal coccidian protozoan that has emerged as an important cause of both epidemic and endemic protracted diarrhea worldwide. Though humans appear to be the only natural hosts; the role of animals as natural reservoir is uncertain but of increasing concern. The present study aimed to study the prevalence of coccidian in different groups such as immunocompromised, clinically apparent immunocompetent and healthy individuals. Also, the study isolates were assessed for heterogeneity among the sequences. Materials and Methods: Stool samples from different groups of patients were collected. The parasite was detected in stool by different diagnostic tools such as light microscopy and nested PCR-restriction fragment length polymorphism using 18S ribosomal RNA as the target gene. Results: The prevalence of C. cayetanensis was 2.4% (19/800) in the present study. The PCR assay amplified Cyclospora cayetanensis DNA in only 89% (17/19) isolates. Further, sequencing revealed no significant difference among the study isolates and the non-primates. Phylogenetic analysis of the study isolates however, formed two clusters. While one cluster showed close evolutionary association with the C. cayetanensis strains, the other cluster showed evolutionary association with the two non-primate species. Conclusion: The methods described here for detection of C. cayetanensis oocysts are simple, efficient, specific, and sensitive and therefore can be effectively applied for laboratory diagnosis and environmental assessment of fresh produce and water sources. Clinicians should include Cyclospora infection in the differential diagnosis of prolonged or relapsing diarrheal illness even in clinically apparent immunocompetent individuals. |
| Databáze: | OpenAIRE |
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